Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> Polycomb Silencing >> PRC1
Overview
Product name
Anti-RING1 antibody
See all RING1 products (7) ...
Description
Rabbit polyclonal to RING1
Specificity
ab32807 specifically detects isoform 1 of RING1 (~42 kDa) as the epitope for ab32807 is not present in isoform 2. We recommend the use of ab32644 for detecting isoform 2 (~39 kDa) of RING1.
Tested applications
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human RING1.
(Peptide available as ab32806.)
Positive control
This antibody gave a positive signal in Human and Mouse Brain Tissue Lysates.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> Polycomb Silencing >> PRC1
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32807 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 2 Images
WB
Application notes
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42,39 kDa).
ab32807 specifically detects isoform 1 of RING1 (~42 kDa) as the epitope for ab32807 is not present in isoform 2.
We recommend the use of ab32644 for detecting isoform 2 (~39 kDa) of RING1.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Contains 1 RING-type zinc finger.
Cellular localization
Nucleus speckle.
Target information above from: UniProt accessionQ06587
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Ring1A antibody
- RING finger protein 1 antibody
- Transcription repressor Ring1A antibody
see all
Database links
- Entrez Gene: 6015 Human
- Entrez Gene: 19763 Mouse
- Entrez Gene: 309626 Rat
- Omim: 602045 Human
- SwissProt: Q06587 Human
- SwissProt: O35730 Mouse
- SwissProt: Q6MGB6 Rat
- Unigene: 727565 Human
see all
Anti-RING1 antibody images:
Western blot - Anti-RING1 antibody (ab32807)
Anti-RING1 antibody (ab32807) at 1 µg/ml + Human Brain Whole Tissue Lysate at 20 µg
Secondary
IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size : 42,39 kDa
Observed band size : 42 kDa (why is the actual band size different from the predicted?)
ab32807 specifically detects isoform 1 of RING1 which is predicted to be ~42 kDa.
The epitope for ab32807 is not present in isoform 2 (the ~39 kDa form).
An alternative antibody is available (ab32644) which does detect isoform 2 of RING1.
Western blot - RING1 antibody (ab32807)
Anti-RING1 antibody (ab32807) at 1 µg/ml + Brain (Mouse) Tissue Lysate at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 42,39 kDa
Observed band size : 42 kDa (why is the actual band size different from the predicted?)
Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
ab32807 specifically detects isoform 1 of RING1 which is predicted to be ~42 kDa.
The epitope for ab32807 is not present in isoform 2 (the ~39 kDa form).
References for Anti-RING1 antibody (ab32807)
This product has been referenced in:
- Vandamme Jet al. Interaction Proteomics Analysis of Polycomb Proteins Defines Distinct PRC1 Complexes in Mammalian Cells. Mol Cell Proteomics 10:M110.002642 (2011). WB; Human.Read more (PubMed: 21282530) »
See 1 publication for this product
Publishing research using ab32807? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"