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Products:Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> Polycomb Silencing >> PRC1
Overview
Product name
Anti-RING1 antibody
See all RING1 products (7) ...
Description
Mouse monoclonal to RING1
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment: NKECPTCRKK LVSKRSLRPD PNFDALISKI YPSREEYEAH QDRVLIRLSR LHNQQALSSS IEEGLRMQAM HRAQRVRRPI PGSDQTTTMS , corresponding to amino acids 81-171 of Human RING1
NKECPTCRKK LVSKRSLRPD PNFDALISKI YPSREEYEAH QDRVLIRLSR LHNQQALSSS IEEGLRMQAM HRAQRVRRPI PGSDQTTTMS
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG2b
Light chain type
kappa
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> Polycomb Silencing >> PRC1
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Western blot - RING1 antibody (ab57985)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab57985 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Contains 1 RING-type zinc finger.
Cellular localization
Nucleus speckle.
Target information above from: UniProt accessionQ06587
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Ring1A antibody
- RING finger protein 1 antibody
- Transcription repressor Ring1A antibody
see all
Database links
- Entrez Gene: 6015 Human
- Omim: 602045 Human
- SwissProt: Q06587 Human
- Unigene: 727565 Human
Anti-RING1 antibody images:
Western blot - RING1 antibody (ab57985)
Western blot against tagged recombinant protein immunogen using ab57985 RING1 antibody at 1ug/ml. Predicted band size of immunogen is 36 kDa
References for Anti-RING1 antibody (ab57985)
ab57985 has not yet been referenced specifically in any publications.
Publishing research using ab57985? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"