![Western blot - RIP antibody [7H10] (ab72139) image](#ab72139_1.bmp)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIP antibody [7H10] (ab72139) image](#RIP-Primary-antibodies-ab72139-2.jpg)
![Flow Cytometry - Anti-RIP antibody [7H10] (ab72139) image](#RIP-Primary-antibodies-ab72139-3.jpg)
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Products:Cell Biology >> Apoptosis >> Intracellular >> Kinases
Overview
Product name
Anti-RIP antibody [7H10]
See all RIP products (6) ...
Description
Mouse monoclonal [7H10] to RIP
Tested applications
Flow Cyt, WB, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Recombinant full length human RIP protein purified from E.coli (His/ABD-RIP)
Positive control
HeLa, K562 and SK-N-MC cell lysates
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.03% Sodium Azide
Constituents: 50% Glycerol, 0.01% BSA, HEPES, 0.15M Sodium chloride
Concentration
Concentration information loading...
Clonality
Monoclonal
Clone number
7H10
Isotype
IgG2b
Light chain type
kappa
Research areas
Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> RIP
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Cell Biology >> Apoptosis >> Intracellular >> Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab72139 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: 1/50 - 1/100.
WB: 1/500 - 1/2000.Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).
IP: Use at an assay dependent concentration. (Use at a concentration of 1 µl/ 400 µl of lysate)
Target
Function
Essential adapter molecule for the activation of NF-kappa-B. Following different upstream signals (binding of inflammatory cytokines, stimulation of pathogen recognition receptors, or DNA damage), particular RIPK1-containing complexes are formed, initiating a limited number of cellular responses. Upon TNFA stimulation RIPK1 is recruited to a TRADD-TRAF complex initiated by TNFR1 trimerization. There, it is ubiquitinated via 'Lys-63'-link chains, inducing its association with the IKK complex, and its activation through NEMO binding of polyubiquitin chains.
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 1 death domain.
Contains 1 protein kinase domain.
Post-translational
modifications
Proteolytically cleaved by caspase-8 during TNF-induced apoptosis. Cleavage abolishes NF-kappa-B activation and enhances pro-apototic signaling through the TRADD-FADD interaction.
Autophosphorylated on serine and threonine residues.
Ubiquitinated by 'Lys-11'-, 'Lys-48'-, 'Lys-63'- and linear-linked type ubiquitin. Polyubiquitination with 'Lys-63'-linked chains by TRAF2 induces association with the IKK complex. Deubiquitination of 'Lys-63'-linked chains and polyubiquitination with 'Lys-48'-linked chains by TNFAIP3 leads to RIPK1 proteasomal degradation and consequently to the termination of the TNF- or Linear polyubiquitinated; the head-to-tail polyubiquitination is mediated by the LUBAC complex. LPS-mediated activation of NF-kappa-B. Also ubiquitinated with 'Lys-11'-linked chains.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ13546
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cell death protein RIP antibody
- FLJ39204 antibody
- OTTHUMP00000039163 antibody
see all
Database links
- Entrez Gene: 8737 Human
- Entrez Gene: 19766 Mouse
- Entrez Gene: 306886 Rat
- Omim: 603453 Human
- SwissProt: Q13546 Human
- SwissProt: Q60855 Mouse
- Unigene: 519842 Human
- Unigene: 374799 Mouse
see all
Anti-RIP antibody [7H10] images:
Western blot - RIP antibody [7H10] (ab72139)
![Western blot - RIP antibody [7H10] (ab72139)](/ps/datasheet/Images/72/ab72139/ab72139_1.bmp)
All lanes : Anti-RIP antibody [7H10] (ab72139) at 1/500 dilution
Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : SK-N-MC cell lysate
Predicted band size : 76 kDa
Observed band size : 76 kDa
Additional bands at : 57 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIP antibody [7H10] (ab72139)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIP antibody [7H10] (ab72139)](/ps/datasheet/images/72/ab72139/RIP-Primary-antibodies-ab72139-2.jpg)
IHC image of ab72139 staining in Breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72139, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Flow Cytometry - Anti-RIP antibody [7H10] (ab72139)
![Flow Cytometry - Anti-RIP antibody [7H10] (ab72139)](/ps/datasheet/images/72/ab72139/RIP-Primary-antibodies-ab72139-3.jpg)
Overlay histogram showing HEK293 cells stained with ab72139 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab72139, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
References for Anti-RIP antibody [7H10] (ab72139)
ab72139 has not yet been referenced specifically in any publications.
Publishing research using ab72139? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"