RIP antibody (ab10427)

Product Name 

RIP antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to RIP

Immunogen 

Synthetic peptide: KWVMREGIKGATVGKLA conjugated to BSA by a Glutaraldehyde linker, corresponding to amino acids 634-650 of Human RIPK1.
BLAST 'KWVMREGIKGATVGKLA' with or

Reacts with 
(species key)

Hu

Specificity 

By immunoblotting, the antibody reacts specifically with RIP (single band at 74 kD).
Staining of RIP band by immunoblotting is specifically inhibited by the RIP peptide (amino acid residues 634-650).

Epitope 

Anti-RIP recognizes an epitope located on the C-terminal region of RIP.

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
WB: 1/400. Detects a band of approximately 74 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Jurkat whole cell lysate.

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> RIP
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Cell Biology >> Apoptosis >> Intracellular >> Kinases

Relevance 

RIP (Receptor Interacting Protein) is a 74 kD Ser/Thr kinase which interacts with CD95 (Fas/APO1) receptor and the tumor necrosis factor receptor (TNFR1). It is a cell death domain adapter protein which can bind to the adapter proteins TRADD, RAID (CRADD) and TRAF2. RIP contains an N terminal region with homology to protein kinases, an intermediate domain capable of association with MAPKKK and a C terminal region containing an intracellular death domain motif. RIP activates both p38 MAP Kinase and SAPK families. In vitro, RIP induces apoptosis, as well as SAPK/JNK and NF-kB activation. NF-kB activation through TRADD, TRAF2 and RIP can be triggered also by DR3/APO3 upon activation with APO3/Tweak ligand. DR4 and DR5 also use FADD, TRADD, and RIP in their signal transduction pathways. RIP deficient mice fail to thrive, displaying extensive apoptosis in both lymphoid and adipose tissues and dying at 1-3 days of age. RIP possesses kinase activity as it autophosphorylates itself on serine and threonine residues.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.01M PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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