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Western blot - RIP antibody (ab2035)
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Product Name
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RIP antibody
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See all RIP antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to RIP
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Immunogen
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Synthetic peptide conjugated to KLH, corresponding to a region between amino acids 420-433 of Human RIPK1.
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Reacts with
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Hu
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/1000. Detects a band of approximately 76 kDa (predicted molecular weight: 80.5 kDa).
ELISA: Positive result against immunising peptide.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human muscle lysate.
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Cellular localization
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Cytoplasmic
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Research areas
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Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> RIP
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Cell Biology >> Apoptosis >> Intracellular >> Kinases
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Relevance
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RIP (Receptor Interacting Protein) is a 74 kD Ser/Thr kinase which interacts with CD95 (Fas/APO1) receptor and the tumor necrosis factor receptor (TNFR1). It is a cell death domain adapter protein which can bind to the adapter proteins TRADD, RAID (CRADD) and TRAF2. RIP contains an N terminal region with homology to protein kinases, an intermediate domain capable of association with MAPKKK and a C terminal region containing an intracellular death domain motif. RIP activates both p38 MAP Kinase and SAPK families. In vitro, RIP induces apoptosis, as well as SAPK/JNK and NF-kB activation. NF-kB activation through TRADD, TRAF2 and RIP can be triggered also by DR3/APO3 upon activation with APO3/Tweak ligand. DR4 and DR5 also use FADD, TRADD, and RIP in their signal transduction pathways. RIP deficient mice fail to thrive, displaying extensive apoptosis in both lymphoid and adipose tissues and dying at 1-3 days of age. RIP possesses kinase activity as it autophosphorylates itself on serine and threonine residues.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: PBS, Sucrose
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Form
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Liquid
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Concentration
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2.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this RIP antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for RIP antibody images, references, products related to ab2035 and other tools.
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RIP antibody images:
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 Western blot - RIP antibody (ab2035)
Predicted band size : 80.5 kDa
ab2035 at 1/1000 dilution staining approximately 76kDa band of human RIPK1 in human muscle lysate by Western blot (ECL).
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Search PubMed (MEDLINE) for references to RIP
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RIP antibody - more information
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Customer reviews (feedback) regarding RIP antibody |
Customer FAQs regarding RIP antibody |
Protocols for RIP antibody |
Price and availability of products related to RIP antibody |
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RIP antibody - related products:
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RIP antibody - other tools:
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Contact Abcam with a Technical Enquiry about RIP antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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