Feedback Form

Product Name 

RIP antibody

 

See all RIP antibodies (4)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to RIP

Immunogen 

Synthetic peptide mapping to the C-terminus of human RIP

Reacts with 
(species key)

Hu

Specificity 

This antibody detects a 74 kDa protein, corresponding to the apparent molecular weight of RIP on SDS-PAGE immunoblots.

Tested applications 
(see key)

WB


Abreviews 

Customer reviews
(see key)


Read about Abreviews

Submit an Abreview

Be the first to review this antibody and earn 220 Abpoints.


Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 1 - 2 µg/ml.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> RIP
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Cell Biology >> Apoptosis >> Intracellular >> Kinases

Relevance 

RIP (Receptor Interacting Protein) is a 74 kD Ser/Thr kinase which interacts with CD95 (Fas/APO1) receptor and the tumor necrosis factor receptor (TNFR1). It is a cell death domain adapter protein which can bind to the adapter proteins TRADD, RAID (CRADD) and TRAF2. RIP contains an N terminal region with homology to protein kinases, an intermediate domain capable of association with MAPKKK and a C terminal region containing an intracellular death domain motif. RIP activates both p38 MAP Kinase and SAPK families. In vitro, RIP induces apoptosis, as well as SAPK/JNK and NF-kB activation. NF-kB activation through TRADD, TRAF2 and RIP can be triggered also by DR3/APO3 upon activation with APO3/Tweak ligand. DR4 and DR5 also use FADD, TRADD, and RIP in their signal transduction pathways. RIP deficient mice fail to thrive, displaying extensive apoptosis in both lymphoid and adipose tissues and dying at 1-3 days of age. RIP possesses kinase activity as it autophosphorylates itself on serine and threonine residues.

 

Alternative names RIP antibody (ab2563)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

8737    (Human)

Omim

    

603453    (Human)

SwissProt

    

Q13546    (Human)

Unigene

    

390758    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

PBS pH7.2 with 50% glycerol, 1% BSA and 0.02% sodium azide

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this RIP antibody is on this datasheet. But please do contact us if you would like any reassurance!



References for RIP antibody (ab2563)

Specific publication references   This product has been used in:

Stanger BZ et al. RIP: a novel protein containing a death domain that interacts with Fas/APO-1 (CD95) in yeast and causes cell death. Cell 81:513-23 (1995). PubMed: 7538908

If you publish research using ab2563 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to RIP

 

RIP antibody - more information

Customer reviews (feedback) regarding RIP antibody

Customer FAQs regarding RIP antibody

Protocols for RIP antibody

Price and availability of products related to RIP antibody

 

RIP antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Compatible Secondaries for RIP antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other RIP Antibodies

See all RIP antibodies (4)...

 

RIP antibody - other tools:

Contact Abcam with a Technical Enquiry about RIP antibody

 

All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

©1998-2010 Abcam plc. All rights reserved.