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Products:Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Estrogen
Overview
Product name
Anti-RIP140 antibody
See all RIP140 products (6) ...
Description
Rabbit polyclonal to RIP140
Tested applications
IP, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: GNPTKGRVKKKEKTPLRD, corresponding to amino acids 718-735 of Human RIP140.
GNPTKGRVKK KEKTPLRD
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS, 1 mg/ml BSA and 0.05% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Primary antibody notes
Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Receptor Interacting Protein 140 (RIP 140) has been shown to enhance estrogen receptor transcriptional activity through the AF2 activation domain in an agonist dependent manner and has also been implicated to be involved in other steroid receptor pathways, including the retinoid pathway. RIP 140 has recently been shown to interact with peroxisome proliferator-activated receptor-alpha (PPAR alpha) and has been proposed to competitively inhibit PPAR alpha interaction with Steroid Receptor Coactivator-1 (SRC-1).
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Co-factors
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Estrogen
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Estrogen
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Western blot - RIP140 antibody (ab3425)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab3425 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution.
WB: Use a concentration of 2 µg/mlDetects a band of approximately 140 kDa (predicted molecular weight: 129 kDa).
Target
Relevance
RIP140 is a nuclear protein that specifically interacts with the hormone-dependent activation domain AF2 of nuclear receptors. Also known as nuclear receptor interacting protein 1 (NRIP1), this protein modulates transcriptional activity of the estrogen receptor.
Cellular localization
Nuclear
Alternative names
- NRIP 1 antibody
- NRIP1 antibody
- Nuclear factor RIP 140 antibody
see all
Database links
- Entrez Gene: 8204 Human
- Omim: 602490 Human
- SwissProt: P48552 Human
- Unigene: 155017 Human
Anti-RIP140 antibody images:
Western blot - RIP140 antibody (ab3425)
Predicted band size : 129 kDa
Western blot of recombinant human RIP 140 using ab3425.
References for Anti-RIP140 antibody (ab3425)
This product has been referenced in:
- Ljubicic Vet al. Chronic AMPK activation evokes the slow, oxidative myogenic program and triggers beneficial adaptations in mdx mouse skeletal muscle. Hum Mol Genet : (2011). WB; Mouse.Read more (PubMed: 21659335) »
- Frier BCet al. Reductions in RIP140 are not required for exercise- and AICAR-mediated increases in skeletal muscle mitochondrial content. J Appl Physiol 111:688-95 (2011). WB; Human, Rat.Read more (PubMed: 21700896) »
See all 5 publications for this product
Publishing research using ab3425? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"