![Western blot - RIP2 antibody [EPR4224(2)] (ab108971) image](#RIP2-Primary-antibodies-ab108971-1.jpg)
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Product name
Anti-RIP2 antibody [EPR4224(2)]
See all RIP2 products (10) ...
Description
Rabbit monoclonal [EPR4224(2)] to RIP2
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide corresponding to residues in Human RIP2 (UniProt O43353).
Positive control
HeLa, K562, THP1 and Raji cell lysates
General notes
Produced under U.S. Patent No. 5,675,063
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR4224(2)
Isotype
IgG1
Research areas
Metabolism >> Types of disease >> Obesity
Immunology >> Innate Immunity >> TLR Signaling
Cancer >> Invasion/microenvironment >> Apoptosis >> Other
Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> RIP
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> CARD Family
Cell Biology >> Apoptosis >> Intracellular >> Kinases
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Western blot - RIP2 antibody [EPR4224(2)] (ab108971)
![Western blot - RIP2 antibody [EPR4224(2)] (ab108971) image](/ps/datasheet/images/108/ab108971/RIP2-Primary-antibodies-ab108971-1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab108971 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/10000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
Application notes
Is unsuitable for ,Flow Cyt,ICC,IHC-P or IP.
Target
Function
Activates pro-caspase-1 and pro-caspase-8. Potentiates CASP8-mediated apoptosis. Activates NF-kappa-B.
Tissue specificity
Detected in heart, brain, placenta, lung, peripheral blood leukocytes, spleen, kidney, testis, prostate, pancreas and lymph node.
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 1 CARD domain.
Contains 1 protein kinase domain.
Post-translational
modifications
Autophosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination catalyzed by ITCH.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionO43353
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CARD 3 antibody
- CARD carrying kinase antibody
- CARD containing ICE associated kinase antibody
see all
Database links
- Entrez Gene: 8767 Human
- Entrez Gene: 192656 Mouse
- Entrez Gene: 362491 Rat
- Omim: 603455 Human
- SwissProt: O43353 Human
- SwissProt: P58801 Mouse
- Unigene: 103755 Human
- Unigene: 112765 Mouse
see all
Anti-RIP2 antibody [EPR4224(2)] images:
Western blot - RIP2 antibody [EPR4224(2)] (ab108971)
All lanes : Anti-RIP2 antibody [EPR4224(2)] (ab108971) at 1/1000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : K562 cell lysates
Lane 3 : THP1 cell lysates
Lane 4 : Raji cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 61 kDa
Observed band size : 61 kDa
References for Anti-RIP2 antibody [EPR4224(2)] (ab108971)
ab108971 has not yet been referenced specifically in any publications.
Publishing research using ab108971? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"