Loading...
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Anti-RNA Helicase A antibody
See all RNA Helicase A products (8) ...
Mouse monoclonal to RNA Helicase A
IP, WB, IHC-P, ICC/IFmore details
Reacts with
Human
Recombinant fragment: MGDVKNFLYA WCGKRKMTPT YEIRAVGNKN RQKFMCEVQV EGYNYTGMGN STNKKDAQSN AARDFVNYLV RINEIKSEEV PAFGVASPPP , corresponding to amino acids 1-91 of Human RNA Helicase A
MGDVKNFLYA WCGKRKMTPT YEIRAVGNKN RQKFMCEVQV EGYNYTGMGN STNKKDAQSN AARDFVNYLV RINEIKSEEV PAFGVASPPP
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: None
PBS, pH 7.2
Concentration information loading...
Protein G purified
Monoclonal
IgG1
kappa
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Nuclear Pore Complex
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Our Abpromise guarantee covers the use of ab54593 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at a concentration of 10 µg/ml.
IHC-P: Use at a concentration of 3 µg/ml.
IP: Use at an assay dependent dilution (PMID 19229320).
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 141 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure may subsequently influence interactions with proteins or other nucleic acids. Functions as a transcriptional activator. Component of the CRD-mediated complex that promotes MYC mRNA stability.
Belongs to the DEAD box helicase family. DEAH subfamily.
Contains 2 DRBM (double-stranded RNA-binding) domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
The MTAD domain mediates interaction with the RNA polymerase II holoenzyme. The NTD domain is necessary and sufficient for nucleo-cytoplasmic shuttling and interaction with HRMT1L2 and SMN1.
Methylated. HRMT1L2 mediated methylation of undefined Arg residues in the NTD is required for nuclear localization.
May be phosphorylated by PRKDC/XRCC7. Phosphorylated upon DNA damage, probably by ATM or ATR.
Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Can shuttle between nucleus and cytoplasm.
Target information above from: UniProt accessionQ08211
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
IHC-P - RNA Helicase A antibody (ab54593)

RNA Helicase A antibody (ab54593) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human testis.
Western blot - RNA Helicase A antibody (ab54593)

RNA Helicase A antibody (ab54593) at 1ug/lane + HeLa cell lysate at 25ug/lane.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RNA Helicase A antibody (ab54593)

ab54593 at 10 µg/ml staining RNA Helicase A in human Hela cells by Immunocytochemistry / Immunofluorescence.
This product has been referenced in:
See all 5 publications for this product
Publishing research using ab54593? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:

RNA Helicase A antibody (ab54593) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human testis.

RNA Helicase A antibody (ab54593) at 1ug/lane + HeLa cell lysate at 25ug/lane.

ab54593 at 10 µg/ml staining RNA Helicase A in human Hela cells by Immunocytochemistry / Immunofluorescence.
2
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Call 01223 696 000 or contact us
