Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Overview
Product name
Anti-RNA Helicase A antibody
See all RNA Helicase A products (8) ...
Description
Mouse monoclonal to RNA Helicase A
Tested applications
IP, WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment: MGDVKNFLYA WCGKRKMTPT YEIRAVGNKN RQKFMCEVQV EGYNYTGMGN STNKKDAQSN AARDFVNYLV RINEIKSEEV PAFGVASPPP , corresponding to amino acids 1-91 of Human RNA Helicase A
MGDVKNFLYA WCGKRKMTPT YEIRAVGNKN RQKFMCEVQV EGYNYTGMGN STNKKDAQSN AARDFVNYLV RINEIKSEEV PAFGVASPPP
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG1
Light chain type
kappa
Research areas
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Nuclear Pore Complex
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab54593 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP
- 2 Images
IHC-P
ICC/IF
Application notes
ICC/IF: Use at a concentration of 10 µg/ml.
IHC-P: Use at a concentration of 3 µg/ml.
IP: Use at an assay dependent dilution (PMID 19229320).
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 141 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure may subsequently influence interactions with proteins or other nucleic acids. Functions as a transcriptional activator. Component of the CRD-mediated complex that promotes MYC mRNA stability.
Sequence similarities
Belongs to the DEAD box helicase family. DEAH subfamily.
Contains 2 DRBM (double-stranded RNA-binding) domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Domain
The MTAD domain mediates interaction with the RNA polymerase II holoenzyme. The NTD domain is necessary and sufficient for nucleo-cytoplasmic shuttling and interaction with HRMT1L2 and SMN1.
Post-translational
modifications
Methylated. HRMT1L2 mediated methylation of undefined Arg residues in the NTD is required for nuclear localization.
May be phosphorylated by PRKDC/XRCC7. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Can shuttle between nucleus and cytoplasm.
Target information above from: UniProt accessionQ08211
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ATP dependent RNA helicase A antibody
- ATP-dependent RNA helicase A antibody
- DDX 9 antibody
see all
Database links
- Entrez Gene: 1660 Human
- Omim: 603115 Human
- SwissProt: Q08211 Human
- Unigene: 191518 Human
Anti-RNA Helicase A antibody images:
IHC-P - RNA Helicase A antibody (ab54593)
RNA Helicase A antibody (ab54593) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human testis.
Western blot - RNA Helicase A antibody (ab54593)
RNA Helicase A antibody (ab54593) at 1ug/lane + HeLa cell lysate at 25ug/lane.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RNA Helicase A antibody (ab54593)
ab54593 at 10 µg/ml staining RNA Helicase A in human Hela cells by Immunocytochemistry / Immunofluorescence.
References for Anti-RNA Helicase A antibody (ab54593)
This product has been referenced in:
- Jaganathan Set al. A functional nuclear epidermal growth factor receptor, SRC and stat3 heteromeric complex in pancreatic cancer cells. PLoS One 6:e19605 (2011). WB; Human.Read more (PubMed: 21573184) »
- Jin Jet al. Evidence that Lin28 stimulates translation by recruiting RNA helicase A to polysomes. Nucleic Acids Res 39:3724-34 (2011). WB; Human.Read more (PubMed: 21247876) »
See all 5 publications for this product
Publishing research using ab54593? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"