Anti-RNA Helicase A antibody (ab54593)

Overview

• Product nameAnti-RNA Helicase A antibodySee all RNA Helicase A primary antibodies ...
• Description
  Mouse monoclonal to RNA Helicase A
• Tested applicationsIP, WB, IHC-P, Flow Cyt, ICC/IF more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant fragment: MGDVKNFLYA WCGKRKMTPT YEIRAVGNKN RQKFMCEVQV EGYNYTGMGN STNKKDAQSN AARDFVNYLV RINEIKSEEV PAFGVASPPP , corresponding to amino acids 1-91 of Human RNA Helicase A

  Run BLAST with Run BLAST with

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  PBS, pH 7.2
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • RNA Processing
  • Other

  • Epigenetics and Nuclear Signaling
  • Chromatin Binding Proteins
  • DNA / RNA binding

  • Epigenetics and Nuclear Signaling
  • Nuclear Signaling Pathways
  • Nuclear Receptors
  • Nuclear Pore Complex

Applications

Target

• FunctionUnwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure may subsequently influence interactions with proteins or other nucleic acids. Functions as a transcriptional activator. Component of the CRD-mediated complex that promotes MYC mRNA stability.
• Sequence similaritiesBelongs to the DEAD box helicase family. DEAH subfamily.
  Contains 2 DRBM (double-stranded RNA-binding) domains.
  Contains 1 helicase ATP-binding domain.
  Contains 1 helicase C-terminal domain.
• DomainThe MTAD domain mediates interaction with the RNA polymerase II holoenzyme. The NTD domain is necessary and sufficient for nucleo-cytoplasmic shuttling and interaction with HRMT1L2 and SMN1.
• Post-translational
  modificationsMethylated. HRMT1L2 mediated methylation of undefined Arg residues in the NTD is required for nuclear localization.
  May be phosphorylated by PRKDC/XRCC7. Phosphorylated upon DNA damage, probably by ATM or ATR.
• Cellular localizationNucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Can shuttle between nucleus and cytoplasm.

Target information above from: UniProt accession Q08211 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1660 Human
  • Omim: 603115 Human
  • SwissProt: Q08211 Human
  • Unigene: 191518 Human

• Alternative names
  ATP dependent RNA helicase A antibodyATP-dependent RNA helicase A antibodyDDX 9 antibody

  DDX9 antibodyDEAD/H (Asp Glu Ala Asp/His) box polypeptide 9 antibodyDEAD/H box 9 antibodyDEAD/H box polypeptide 9 antibodyDEAH (Asp Glu Ala His) box polypeptide 9 antibodyDEAH box polypeptide 9 antibodyDEAH box protein 9 antibodyDHX 9 antibodyDHX9 antibodyDHX9_HUMAN antibodyLeukophysin antibodyLKP antibodyNDH 2 antibodyNDH II antibodyNDH2 antibodyNDHII antibodyNuclear DNA helicase II antibodyRHA antibody

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Anti-RNA Helicase A antibody images

• 

  IHC-P - RNA Helicase A antibody (ab54593)
  RNA Helicase A antibody (ab54593) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human testis.
• 

  Western blot - RNA Helicase A antibody (ab54593)
  
  
  Predicted band size : 141 kDa
  RNA Helicase A antibody (ab54593) at 1ug/lane + HeLa cell lysate at 25ug/lane.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RNA Helicase A antibody (ab54593)

  ab54593 at 10 µg/ml staining RNA Helicase A in human Hela cells by Immunocytochemistry / Immunofluorescence.

• 

  Flow Cytometry-Anti-RNA Helicase A antibody(ab54593)
  Overlay histogram showing HeLa cells stained with ab54593 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54593, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.