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IHC-P - RNA Helicase A antibody (ab54593)
IHC-P - RNA Helicase A antibody (ab54593)
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Western blot - RNA Helicase A antibody (ab54593)
Western blot - RNA Helicase A antibody (ab54593)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RNA Helicase A antibody (ab54593)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RNA Helicase A antibody (ab54593)
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Product Name 

RNA Helicase A antibody

 

See all RNA Helicase A antibodies (7)...

Product type 

Primary antibodies

Description 

Mouse monoclonal to RNA Helicase A

Immunogen 

Recombinant fragment: MGDVKNFLYA WCGKRKMTPT YEIRAVGNKN RQKFMCEVQV EGYNYTGMGN STNKKDAQSN AARDFVNYLV RINEIKSEEV PAFGVASPPP , corresponding to amino acids 1-91 of Human RNA Helicase A
BLAST 'MGDVKNFLYA WCGKRKMTPT YEIRAVGNKN RQKFMCEVQV EGYNYTGMGN STNKKDAQSN AARDFVNYLV RINEIKSEEV PAFGVASPPP ' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ICC/IF, IHC-P, IP, WB


Abreviews 

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Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at a concentration of 10 µg/ml.
IHC-P: Use at a concentration of 3 µg/ml.
IP: Use at an assay dependent dilution (PMID 19229320).
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 141 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic and nuclear; nucleolar. Can shuttle between nucleus and cytoplasm.

Research areas 

Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Nuclear Pore Complex
Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other

Relevance 

RNA Helicase A unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure may subsequently influence interactions with proteins or other nucleic acids. It functions as a transcriptional activator.

It binds MBD2, HRMT1L2/PRMT1, and RELA. It may act to directly link BRCA1, CREBBP or SMN1 and the RNA polymerase II complex. It can also interact with XRCC5 and with TOP2A in a RNA dependent manner; these interactions may be indirect. Interaction with TOP2A is promoted by UBC9 (referenced from swissprot).

 

Alternative names RNA Helicase A antibody (ab54593)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

1660    (Human)

GeneCard

    

GC01P181075    (Human)

Omim

    

603115    (Human)

SwissProt

    

Q08211    (Human)

Unigene

    

191518    (Human)

Raised in 

Mouse

Clonality 

Monoclonal

Isotype 

IgG1

Light chain type 

kappa

Purity 

Protein G purified

Storage buffer 

Preservative: None
PBS, pH 7.2

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this RNA Helicase A antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for RNA Helicase A antibody images, references, products related to ab54593 and other tools.

 

RNA Helicase A antibody images:

Product image  IHC-P - RNA Helicase A antibody (ab54593)

IHC-P - RNA Helicase A antibody (ab54593)

RNA Helicase A antibody (ab54593) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human testis.

 

Product image  Western blot - RNA Helicase A antibody (ab54593)

Western blot - RNA Helicase A antibody (ab54593)



Predicted band size : 141 kDa


RNA Helicase A antibody (ab54593) at 1ug/lane + HeLa cell lysate at 25ug/lane.

 

Product image  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RNA Helicase A antibody (ab54593)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RNA Helicase A antibody (ab54593)

ab54593 at 10 µg/ml staining RNA Helicase A in human Hela cells by Immunocytochemistry / Immunofluorescence.

 

References for RNA Helicase A antibody (ab54593)

Specific publication references   This product has been used in:

Sadler AJ et al. An antiviral response directed by PKR phosphorylation of the RNA helicase A. PLoS Pathog 5:e1000311 (2009). IP; Human. PubMed: 19229320

If you publish research using ab54593 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to RNA Helicase A

 

RNA Helicase A antibody - more information

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RNA Helicase A antibody - related products:

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