RNA Helicase A antibody (ab54593)

Product Name 

RNA Helicase A antibody

Product type 

Primary antibodies

Description 

Mouse monoclonal to RNA Helicase A

Immunogen 

Recombinant fragment: MGDVKNFLYA WCGKRKMTPT YEIRAVGNKN RQKFMCEVQV EGYNYTGMGN STNKKDAQSN AARDFVNYLV RINEIKSEEV PAFGVASPPP , corresponding to amino acids 1-91 of Human RNA Helicase A
BLAST 'MGDVKNFLYA WCGKRKMTPT YEIRAVGNKN RQKFMCEVQV EGYNYTGMGN STNKKDAQSN AARDFVNYLV RINEIKSEEV PAFGVASPPP ' with or

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ICC/IF, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at a concentration of 10 µg/ml.
IHC-P: Use at a concentration of 3 µg/ml.
IP: Use at an assay dependent dilution (PMID 19229320).
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 141 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic and nuclear; nucleolar. Can shuttle between nucleus and cytoplasm.

Research areas 

Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Nuclear Pore Complex
Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other

Relevance 

RNA Helicase A unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure may subsequently influence interactions with proteins or other nucleic acids. It functions as a transcriptional activator.

It binds MBD2, HRMT1L2/PRMT1, and RELA. It may act to directly link BRCA1, CREBBP or SMN1 and the RNA polymerase II complex. It can also interact with XRCC5 and with TOP2A in a RNA dependent manner; these interactions may be indirect. Interaction with TOP2A is promoted by UBC9 (referenced from swissprot).

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Isotype 

IgG1

Light chain type 

kappa

Purity 

Protein G purified

Storage buffer 

Preservative: None
PBS, pH 7.2

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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RNA Helicase A antibody images:

References for RNA Helicase A antibody (ab54593)

  This product has been used in:

Sadler AJ et al. An antiviral response directed by PKR phosphorylation of the RNA helicase A. PLoS Pathog 5:e1000311 (2009). IP; Human. PubMed: 19229320

If you publish research using ab54593 please let us know so that we can cite the reference on this datasheet.

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