Overview

  • Product nameAnti-RNA Helicase A antibody
    See all RNA Helicase A primary antibodies
  • Description
    Mouse monoclonal to RNA Helicase A
  • Tested applicationsIP, WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: MGDVKNFLYA WCGKRKMTPT YEIRAVGNKN RQKFMCEVQV EGYNYTGMGN STNKKDAQSN AARDFVNYLV RINEIKSEEV PAFGVASPPP , corresponding to amino acids 1-91 of Human RNA Helicase A

Properties

Applications

Our Abpromise guarantee covers the use of ab54593 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution. (PMID 19229320)
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 141 kDa.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 1µg for 106 cells.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionUnwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure may subsequently influence interactions with proteins or other nucleic acids. Functions as a transcriptional activator. Component of the CRD-mediated complex that promotes MYC mRNA stability.
  • Sequence similaritiesBelongs to the DEAD box helicase family. DEAH subfamily.
    Contains 2 DRBM (double-stranded RNA-binding) domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • DomainThe MTAD domain mediates interaction with the RNA polymerase II holoenzyme. The NTD domain is necessary and sufficient for nucleo-cytoplasmic shuttling and interaction with HRMT1L2 and SMN1.
  • Post-translational
    modifications
    Methylated. HRMT1L2 mediated methylation of undefined Arg residues in the NTD is required for nuclear localization.
    May be phosphorylated by PRKDC/XRCC7. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Can shuttle between nucleus and cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP dependent RNA helicase A antibody
    • ATP-dependent RNA helicase A antibody
    • DDX 9 antibody
    • DDX9 antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 9 antibody
    • DEAD/H box 9 antibody
    • DEAD/H box polypeptide 9 antibody
    • DEAH (Asp Glu Ala His) box polypeptide 9 antibody
    • DEAH box polypeptide 9 antibody
    • DEAH box protein 9 antibody
    • DHX 9 antibody
    • DHX9 antibody
    • DHX9_HUMAN antibody
    • Leukophysin antibody
    • LKP antibody
    • NDH 2 antibody
    • NDH II antibody
    • NDH2 antibody
    • NDHII antibody
    • Nuclear DNA helicase II antibody
    • RHA antibody
    see all

Anti-RNA Helicase A antibody images

  • RNA Helicase A antibody (ab54593) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human testis.


  • Predicted band size : 141 kDa
    RNA Helicase A antibody (ab54593) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab54593 at 10 µg/ml staining RNA Helicase A in human Hela cells by Immunocytochemistry / Immunofluorescence.

  • Overlay histogram showing HeLa cells stained with ab54593 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54593, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-RNA Helicase A antibody (ab54593)

This product has been referenced in:
  • Jefferson M  et al. Host factors that interact with the pestivirus N-terminal protease, Npro, are components of the ribonucleoprotein complex. J Virol 88:10340-53 (2014). ICC/IF . Read more (PubMed: 24965446) »
  • Lin L  et al. Identification of RNA helicase A as a cellular factor that interacts with influenza A virus NS1 protein and its role in the virus life cycle. J Virol 86:1942-54 (2012). WB . Read more (PubMed: 22171255) »

See all 7 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (U2OS cells)
Loading amount 20 µg
Specification U2OS cells
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

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Verified customer

Submitted Dec 19 2008

Application Western blot
Sample African Green Monkey Cell lysate - whole cell (CV-1, COS-7)
Loading amount 20 µg
Specification CV-1, COS-7
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Nov 26 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"