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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Overview
Product name
Anti-RNA Helicase A antibody
See all RNA Helicase A products (8) ...
Description
Rabbit polyclonal to RNA Helicase A
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide corresponding to a region between residue 1220 and the C-terminus (residue 1270) of human RNA Helicase A (CAA71668.1)
Positive control
HeLa and 293T whole cell lysates.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab70777 was affinity purified using an epitope specific to RNA Helicase A immobilized on solid support.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Nuclear Pore Complex
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70777 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
IP: Use at 2-5µg/mg of lysate.
WB: 1/2000 - 1/10000. Detects a band of approximately 141 kDa (predicted molecular weight: 141 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure may subsequently influence interactions with proteins or other nucleic acids. Functions as a transcriptional activator. Component of the CRD-mediated complex that promotes MYC mRNA stability.
Sequence similarities
Belongs to the DEAD box helicase family. DEAH subfamily.
Contains 2 DRBM (double-stranded RNA-binding) domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Domain
The MTAD domain mediates interaction with the RNA polymerase II holoenzyme. The NTD domain is necessary and sufficient for nucleo-cytoplasmic shuttling and interaction with HRMT1L2 and SMN1.
Post-translational
modifications
Methylated. HRMT1L2 mediated methylation of undefined Arg residues in the NTD is required for nuclear localization.
May be phosphorylated by PRKDC/XRCC7. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Can shuttle between nucleus and cytoplasm.
Target information above from: UniProt accessionQ08211
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ATP dependent RNA helicase A antibody
- ATP-dependent RNA helicase A antibody
- DDX 9 antibody
see all
Database links
- Entrez Gene: 1660 Human
- Omim: 603115 Human
- SwissProt: Q08211 Human
- Unigene: 191518 Human
Anti-RNA Helicase A antibody images:
Western blot - RNA Helicase A antibody (ab70777)
All lanes : Anti-RNA Helicase A antibody (ab70777) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size : 141 kDa
Observed band size : 141 kDa
Exposure time : 10 seconds
Immunoprecipitation - RNA Helicase A antibody (ab70777)
Detection of RNA Helicase A by Western Blot of Immunprecipitate. ab70777 at 1µg/ml staining RNA Helicase A in HeLa whole cell lysate immunoprecipitated using ab70777 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.
References for Anti-RNA Helicase A antibody (ab70777)
ab70777 has not yet been referenced specifically in any publications.
Publishing research using ab70777? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"