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Western blot - RNA Helicase A antibody (ab70777)
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Immunoprecipitation - RNA Helicase A antibody (ab70777)
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Product Name
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RNA Helicase A antibody
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See all RNA Helicase A antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to RNA Helicase A
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Immunogen
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Synthetic peptide corresponding to a region between residue 1220 and the C-terminus (residue 1270) of human RNA Helicase A (CAA71668.1)
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IP: Use at 2-5µg/mg of lysate.
WB: 1/2000 - 1/10000. Detects a band of approximately 141 kDa (predicted molecular weight: 141 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa and 293T whole cell lysates.
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Cellular localization
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Cytoplasmic and nuclear; nucleolar. Can shuttle between nucleus and cytoplasm.
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Research areas
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Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Nuclear Pore Complex
Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
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Relevance
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RNA Helicase A unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure may subsequently influence interactions with proteins or other nucleic acids. It functions as a transcriptional activator.
It binds MBD2, HRMT1L2/PRMT1, and RELA. It may act to directly link BRCA1, CREBBP or SMN1 and the RNA polymerase II complex. It can also interact with XRCC5 and with TOP2A in a RNA dependent manner; these interactions may be indirect. Interaction with TOP2A is promoted by UBC9 (referenced from swissprot).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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ab70777 was affinity purified using an epitope specific to RNA Helicase A immobilized on solid support.
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this RNA Helicase A antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for RNA Helicase A antibody images, references, products related to ab70777 and other tools.
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RNA Helicase A antibody images:
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 Western blot - RNA Helicase A antibody (ab70777)
All lanes : RNA Helicase A antibody (ab70777) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size : 141 kDa
Observed band size : 141 kDa
Exposure time : 10 seconds
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Immunoprecipitation - RNA Helicase A antibody (ab70777)
Detection of RNA Helicase A by Western Blot of Immunprecipitate.
ab70777 at 1µg/ml staining RNA Helicase A in HeLa whole cell lysate immunoprecipitated using ab70777 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.
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Search PubMed (MEDLINE) for references to RNA Helicase A
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RNA Helicase A antibody - more information
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Customer reviews (feedback) regarding RNA Helicase A antibody |
Customer FAQs regarding RNA Helicase A antibody |
Protocols for RNA Helicase A antibody |
Price and availability of products related to RNA Helicase A antibody |
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RNA Helicase A antibody - related products:
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RNA Helicase A antibody - other tools:
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