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Products:Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription
Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (ab52208)
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Secondary antibodies
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Immunohistochemistry (Paraffin-embedded sections) - RNA polymerase II (phospho S1619) antibody (ab52208)
This image shows human breast carcinoma tissue stained with ab52208 at a dilution of 1/50 - 1/100. Right hand image: tissue treated with immunising peptide; left hand image: untreated.
Western blot - RNA polymerase II (phospho S1619) antibody (ab52208)
All lanes : Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (ab52208) at 1/500 dilution
Lane 1 : Extracts from COS7 cells treated
with EGF (200ng/ml, 30min) with no immunising peptide
Lane 2 : Extracts from COS7 cells treated
with EGF (200ng/ml, 30min) with immunising peptide
Predicted band size : 217 kDa
Observed band size : 217 kDa
Immunocytochemistry/ Immunofluorescence-RNA polymerase II (phospho S1619) antibody(ab52208)
ICC/IF image of ab52208 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52208, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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