Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S1801) antibody [EP1510Y] (ab76292)

Overview

• Product nameAnti-RNA polymerase II CTD repeat YSPTSPS (phospho S1801) antibody [EP1510Y]See all RNA polymerase II CTD repeat YSPTSPS primary antibodies ...
• Description
  Rabbit monoclonal [EP1510Y] to RNA polymerase II CTD repeat YSPTSPS (phospho S1801)
• SpecificityDetects RNA Polymeraste II phosphorylated at serine 1801.
• Tested applicationsWB, IP, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  A phospho synthetic peptide corresponding to residues surrounding serine 5 of human RNA Polymerase II CTD repeat YSPTSPS.

• Positive controlWB: Human brain nuclear lysate IHC-P: Human breast carcinoma tissue ICC/IF: HeLa cells
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEP1510Y
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Transcription
  • Polymerase associated factors
  • Pol II Transcription
  • Polymerase

  • Epigenetics and Nuclear Signaling
  • Transcription
  • RNA polymerase

Applications

Target

• FunctionDNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Acts as a RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome.
• Sequence similaritiesBelongs to the RNA polymerase beta' chain family.
• Post-translational
  modificationsThe tandem 7 residues repeats in the C-terminal domain (CTD) can be highly phosphorylated. The phosphorylation activates Pol II. Phosphorylation occurs mainly at residues 'Ser-2' and 'Ser-5' of the heptapeptide repeat and is mediated, at least, by CDK7 and CDK9. CDK7 phosphorylation of POLR2A associated with DNA promotes transcription initiation by triggering dissociation from DNA. Phosphorylation also takes place at 'Ser-7' of the heptapepdtide repeat, which is required for efficient transcription of snRNA genes and processing of the transcripts. The phosphorylation state is believed to result from the balanced action of site-specific CTD kinases and phosphatases, and a 'CTD code' that specifies the position of Pol II within the transcription cycle has been proposed.
  Dephosphorylated by the protein phosphatase CTDSP1.
  Ubiquitinated by WWP2 leading to proteasomal degradation (By similarity). Following UV treatment, the elongating form of RNA polymerase II (RNA pol IIo) is ubiquitinated UV damage sites without leading to degradation: ubiquitination is facilitated by KIAA1530/UVSSA and promotes RNA pol IIo backtracking to allow access to the nucleotide excision repair machinery.
  Methylated at Arg-1810 by CARM1. Methylation occurs only when the CTD is hypophosphorylated, and phosphorylation at Ser-1805 and Ser-1808 prevent methylation (in vitro). It is assumed that methylation occurs prior to phosphorylation and transcription initiation. CTD methylation may facilitate the expression of select RNAs.
• Cellular localizationNucleus.

Target information above from: UniProt accession P24928 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5430 Human
  • Entrez Gene: 20020 Mouse
  • Entrez Gene: 363633 Rat
  • Omim: 180660 Human
  • SwissProt: P24928 Human
  • SwissProt: P08775 Mouse
  • Unigene: 270017 Human
  • Unigene: 16533 Mouse

• Alternative names
  B220 antibodyDNA directed RNA polymerase II A antibodyDNA-directed RNA polymerase II largest subunit antibody

  DNA-directed RNA polymerase II largest subunit RNA polymerase II 220 kd subunit antibodyDNA-directed RNA polymerase II subunit A antibodyDNA-directed RNA polymerase II subunit RPB1 antibodyDNA-directed RNA polymerase III largest subunit antibodyhRPB220 antibodyhsRPB1 antibodyMGC75453 antibodyPOLR2 antibodyPOLR2A antibodyPOLRA antibodyPolymerase (RNA) II (DNA directed) polypeptide A 220kDa antibodyPolymerase (RNA) II (DNA directed) polypeptide A antibodyRNA polymerase II subunit B1 antibodyRNA-directed RNA polymerase II subunit RPB1 antibodyRPB1 antibodyRPB1_HUMAN antibodyRPB220 antibodyRPBh1 antibodyRpIILS antibodyRPO2 antibodyRPOL2 antibody

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Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S1801) antibody [EP1510Y] images

• 

  Western blot - RNA polymerase II (phospho S1801) antibody [EP1510Y] (ab76292)
  All lanes : Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S1801) antibody [EP1510Y] (ab76292) at 1/200000 dilution
  
  Lane 1 : Human brain lysates, untreated
  Lane 2 : Human brain lysates treated with AP
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP labelled goat anti-rabbit at 1/1000 dilution
  
  Predicted band size : 217 kDa
  Observed band size : 270 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RNA polymerase II (phospho S1801) antibody [EP1510Y] (ab76292)
  ab76292, at a 1/100 dilution, staining RNA Polymerase II (phospho S1801) in formalin fixed, paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
• 

  Immunocytochemistry/ Immunofluorescence - RNA polymerase II (phospho S1801) antibody [EP1510Y] (ab76292)
  ab76292, at a 1/250 dilution, staining RNA Polymerase II (phospho S1801) in HeLa cells by Immunofluorescence.