Anti-RNA polymerase II RPB1 antibody [EPR1509Y] (ab76123)

Overview

• Product nameAnti-RNA polymerase II RPB1 antibody [EPR1509Y]See all RNA polymerase II RPB1 primary antibodies ...
• Description
  Rabbit monoclonal [EPR1509Y] to RNA polymerase II RPB1
• Tested applicationsWB, ICC, Flow Cyt, ChIP more details
• Species reactivity
  Reacts with: Human
  

  Does not react with

  Mouse, Rat
• Immunogen

  A synthetic peptide corresponding to residues near the N terminus of human RNA polymerase II

• Positive controlHeLa cell lysate.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEPR1509Y
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Transcription
  • Polymerase associated factors
  • Pol II Transcription
  • Polymerase

  • Epigenetics and Nuclear Signaling
  • Transcription
  • RNA polymerase

Applications

Target

• RelevanceRPB1 is a subunit of RNA polymerase II which catalyses transcription of RNA from DNA. RPB1 forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template.
• Cellular localizationNuclear
• Database links
  • Entrez Gene: 5430 Human
  • Omim: 180660 Human
  • SwissProt: P24928 Human

• Alternative names
  B220 antibodyDNA directed RNA polymerase II subunit RPB1 antibodyDNA-directed RNA polymerase II largest subunit antibody

  hRPB220 antibodyhsRPB1 antibodyMGC75453 antibodyPOLR2 antibodyPOLR2A antibodyPOLRA antibodyPolymerase (RNA) II (DNA directed) polypeptide A 220kDa antibodypolymerase (RNA) II (DNA directed) polypeptide A, 220kDa antibodyRNA polymerase II subunit 1 antibodyRNA polymerase II subunit B1 antibodyRP02 antibodyRPB220 antibodyRPBh1 antibodyRpIILS antibodyRPO21 antibodyRPOL2 antibodySUA8 antibody

  see all

Anti-RNA polymerase II RPB1 antibody [EPR1509Y] images

• 

  Western blot - RNA polymerase II antibody [EPR1509Y] (ab76123)
  Anti-RNA polymerase II RPB1 antibody [EPR1509Y] (ab76123) at 1/2000 dilution + HeLa cell lysate at 10 µg
  
  Secondary
  Goat anti-rabbit HRP at 1/2000 dilution
  
  Predicted band size : 217 kDa
  Observed band size : 270 kDa (why is the actual band size different from the predicted?)