|
|
|
|
Immunohistochemistry (Paraffin-embedded sections) - RNA polymerase II (phospho S1619) antibody (ab52208)
(enlarge)
|
|
Western blot - RNA polymerase II (phospho S1619) antibody (ab52208)
(enlarge)
|
|
Immunocytochemistry/ Immunofluorescence-RNA polymerase II (phospho S1619) antibody(ab52208)
(enlarge)
|
|
|
|
|
Product Name
|
RNA polymerase II (phospho S1619) antibody
|
| |
See all RNA polymerase II antibodies (13)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to RNA polymerase II (phospho S1619)
|
|
Immunogen
|
Synthesized phosphopeptide derived from human RNA polymerase II around the phosphorylation site of serine 1619 (P-T-SP-P-S).
|
|
Reacts with
(species key)
|
Hu, Ms, Rat
|
|
Specificity
|
ab52208 detects endogenous levels of RNA polymerase II only when phosphorylated at serine 1619.
|
|
Tested applications
(see key)
|
ELISA, ICC/IF, IHC-P, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ELISA: 1/10000.
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Detects a band of approximately 217 kDa (predicted molecular weight: 217 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Human breast carcinoma tissue; extracts from COS7 cells.
|
|
Cellular localization
|
Nuclear
|
|
Research areas
|
Chromatin and Nuclear Signaling >> Transcription >> RNA polymerase
Chromatin and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> Polymerase
|
|
Relevance
|
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. RNA polymerase II has two physiologically important phosphorylation sites; ser 2 and ser 5. Both sites are found in the heptapeptide repeat YSPTSPS at the C terminal domain.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
|
|
Purification notes
|
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Store at -20°C. Stable for 12 months at -20°C
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this RNA polymerase II (phospho S1619) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for RNA polymerase II (phospho S1619) antibody images, references, products related to ab52208 and other tools.
|
| |
RNA polymerase II (phospho S1619) antibody images:
|
 Immunohistochemistry (Paraffin-embedded sections) - RNA polymerase II (phospho S1619) antibody (ab52208)
This image shows human breast carcinoma tissue stained with ab52208 at a dilution of 1/50 - 1/100. Right hand image: tissue treated with immunising peptide; left hand image: untreated.
|
| |
Western blot - RNA polymerase II (phospho S1619) antibody (ab52208)
All lanes : RNA polymerase II (phospho S1619) antibody (ab52208) at 1/500 dilution
Lane 1 : Extracts from COS7 cells treated
with EGF (200ng/ml, 30min) with no immunising peptide
Lane 2 : Extracts from COS7 cells treated
with EGF (200ng/ml, 30min) with immunising peptide
Predicted band size : 217 kDa
Observed band size : 217 kDa
|
| |
Immunocytochemistry/ Immunofluorescence-RNA polymerase II (phospho S1619) antibody(ab52208)
ICC/IF image of ab52208 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52208, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
|
| |
| |
|
Search PubMed (MEDLINE) for references to RNA polymerase II
|
| |
RNA polymerase II (phospho S1619) antibody - more information
|
Customer reviews (feedback) regarding RNA polymerase II (phospho S1619) antibody |
Customer FAQs regarding RNA polymerase II (phospho S1619) antibody |
Protocols for RNA polymerase II (phospho S1619) antibody |
Price and availability of products related to RNA polymerase II (phospho S1619) antibody |
| |
RNA polymerase II (phospho S1619) antibody - related products:
|
|
|
| |
RNA polymerase II (phospho S1619) antibody - other tools:
|
Contact Abcam with a Technical Enquiry about RNA polymerase II (phospho S1619) antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
