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Western blot - RNA polymerase II (phospho S1801) antibody [EP1510Y] (ab76292)
![Western blot - RNA polymerase II (phospho S1801) antibody [EP1510Y] (ab76292)](/ps/datasheet/images/ab76292_WB_1.jpg)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RNA polymerase II (phospho S1801) antibody [EP1510Y] (ab76292)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RNA polymerase II (phospho S1801) antibody [EP1510Y] (ab76292)](/ps/datasheet/images/ab76292_IHC_1.jpg)
(enlarge)
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Immunocytochemistry/ Immunofluorescence - RNA polymerase II (phospho S1801) antibody [EP1510Y] (ab76292)
![Immunocytochemistry/ Immunofluorescence - RNA polymerase II (phospho S1801) antibody [EP1510Y] (ab76292)](/ps/datasheet/images/ab76292_if_1.jpg)
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Product Name
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RNA polymerase II (phospho S1801) antibody [EP1510Y]
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See all RNA polymerase II antibodies (13)...
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Product type
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Primary antibodies
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Description
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Rabbit monoclonal [EP1510Y] to RNA polymerase II (phospho S1801)
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Immunogen
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A phospho synthetic peptide corresponding to residues surrounding serine 1801 of human RNA Polymerase II.
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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Detects RNA Polymeraste II phosphorylated at serine 1801.
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Tested applications
(see key)
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ICC/IF, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: 1/250 - 1/500.
IHC-P: 1/100 - 1/250. Antigen retrieval is recommended before commencing with IHC staining protocol. Use of HRP conjugated or polymerized HRP secondary antibodies is recommended. Stronger signals have been found using the polymerized HRP secondary.
IP: 1/40.
WB: 1/100,000 - 1/200,000. Detects a band of approximately 217 kDa.
Is unsuitable for Flow Cyt.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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WB: Human brain nuclear lysate
IHC-P: Human breast carcinoma tissue
ICC/IF: HeLa cells
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> RNA polymerase
Chromatin and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> Polymerase
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Relevance
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DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. RNA polymerase II has two physiologically important phosphorylation sites; ser 2 and ser 5. Both sites are found in the heptapeptide repeat YSPTSPS at the C terminal domain.
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RNA polymerase II (phospho S1801) antibody [EP1510Y] (ab76292) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Monoclonal
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Clone number
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EP1510Y
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Isotype
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IgG
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Purity
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Tissue culture supernatant
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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Notes
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Produced under U.S. Patent No. 5,675,063.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this RNA polymerase II (phospho S1801) antibody [EP1510Y] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for RNA polymerase II (phospho S1801) antibody [EP1510Y] images, references, products related to ab76292 and other tools.
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RNA polymerase II (phospho S1801) antibody [EP1510Y] images:
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 Western blot - RNA polymerase II (phospho S1801) antibody [EP1510Y] (ab76292)
All lanes : RNA polymerase II (phospho S1801) antibody [EP1510Y] (ab76292) at 1/200000 dilution
Lane 1 : Human brain lysates, untreated
Lane 2 : Human brain lysates treated with AP
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti-rabbit at 1/1000 dilution
Predicted band size : 217 kDa
Observed band size : 270 kDa (why is the actual band size different from the predicted?)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RNA polymerase II (phospho S1801) antibody [EP1510Y] (ab76292)
ab76292, at a 1/100 dilution, staining RNA Polymerase II (phospho S1801) in formalin fixed, paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
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Immunocytochemistry/ Immunofluorescence - RNA polymerase II (phospho S1801) antibody [EP1510Y] (ab76292)
ab76292, at a 1/250 dilution, staining RNA Polymerase II (phospho S1801) in HeLa cells by Immunofluorescence.
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Search PubMed (MEDLINE) for references to RNA polymerase II
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RNA polymerase II (phospho S1801) antibody [EP1510Y] - more information
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Customer reviews (feedback) regarding RNA polymerase II (phospho S1801) antibody [EP1510Y] |
Customer FAQs regarding RNA polymerase II (phospho S1801) antibody [EP1510Y] |
Protocols for RNA polymerase II (phospho S1801) antibody [EP1510Y] |
Price and availability of products related to RNA polymerase II (phospho S1801) antibody [EP1510Y] |
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RNA polymerase II (phospho S1801) antibody [EP1510Y] - related products:
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RNA polymerase II (phospho S1801) antibody [EP1510Y] - other tools:
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Contact Abcam with a Technical Enquiry about RNA polymerase II (phospho S1801) antibody [EP1510Y] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Western blot - RNA polymerase II (phospho S1801) antibody [EP1510Y] (ab76292)](/index.html?datasheet=76292#ab76292_WB_1.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RNA polymerase II (phospho S1801) antibody [EP1510Y] (ab76292)](/index.html?datasheet=76292#ab76292_IHC_1.jpg)
![Immunocytochemistry/ Immunofluorescence - RNA polymerase II (phospho S1801) antibody [EP1510Y] (ab76292)](/index.html?datasheet=76292#ab76292_if_1.jpg)
