Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody [H5] - ChIP Grade (ab24758)
- Product nameAnti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody [H5] - ChIP GradeSee all RNA polymerase II CTD repeat YSPTSPS primary antibodies ...
- DescriptionMouse monoclonal [H5] to RNA polymerase II CTD repeat YSPTSPS (phospho S2) - ChIP Grade
- SpecificityThe antibody shows some weak reactivity towards unphosphorylated RNA polymerase II, in addition to the phosphorylated protein
- Tested applicationsICC/IF, ChIP, WB, IP, Functional Studies, Purification more details
- Species reactivityReacts with: Mouse, Human, Fruit fly (Drosophila melanogaster), Zebrafish
Predicted to work with: a wide range of other species
Full length native protein - purified phosphorylated form of RNA polymerase II extracted from a transformed cell line.
- General notes
This antibody recognizes the phosphoserine 2 version of pol II.
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: None
- Concentration information loading...
- Primary antibody notes This antibody recognizes the phosphoserine 2 version of pol II.
- Clonality Monoclonal
- Clone numberH5
- Research Areas
Our Abpromise guarantee covers the use of ab24758 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||ICC/IF: Use at an assay dependent dilution.|
|ChIP||ChIP: Use 2-100 µg for µg of chromatin. PubMed: 19363488|
|WB||WB: 1/500. Predicted molecular weight: 217 kDa.|
|Functional Studies||FuncS: Use at an assay dependent dilution.|
|Purification||P: Use at an assay dependent dilution.|
- FunctionDNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Acts as a RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome.
- Sequence similaritiesBelongs to the RNA polymerase beta' chain family.
modificationsThe tandem 7 residues repeats in the C-terminal domain (CTD) can be highly phosphorylated. The phosphorylation activates Pol II. Phosphorylation occurs mainly at residues 'Ser-2' and 'Ser-5' of the heptapeptide repeat and is mediated, at least, by CDK7 and CDK9. CDK7 phosphorylation of POLR2A associated with DNA promotes transcription initiation by triggering dissociation from DNA. Phosphorylation also takes place at 'Ser-7' of the heptapepdtide repeat, which is required for efficient transcription of snRNA genes and processing of the transcripts. The phosphorylation state is believed to result from the balanced action of site-specific CTD kinases and phosphatases, and a 'CTD code' that specifies the position of Pol II within the transcription cycle has been proposed.
Dephosphorylated by the protein phosphatase CTDSP1.
Ubiquitinated by WWP2 leading to proteasomal degradation (By similarity). Following UV treatment, the elongating form of RNA polymerase II (RNA pol IIo) is ubiquitinated UV damage sites without leading to degradation: ubiquitination is facilitated by KIAA1530/UVSSA and promotes RNA pol IIo backtracking to allow access to the nucleotide excision repair machinery.
Methylated at Arg-1810 by CARM1. Methylation occurs only when the CTD is hypophosphorylated, and phosphorylation at Ser-1805 and Ser-1808 prevent methylation (in vitro). It is assumed that methylation occurs prior to phosphorylation and transcription initiation. CTD methylation may facilitate the expression of select RNAs.
- Cellular localizationNucleus.
- Entrez Gene: 32100 Fruit fly (Drosophila melanogaster)
- Entrez Gene: 5430 Human
- Entrez Gene: 20020 Mouse
- Omim: 180660 Human
- SwissProt: P04052 Fruit fly (Drosophila melanogaster)
- SwissProt: P24928 Human
- SwissProt: P08775 Mouse
- Unigene: 2925 Fruit fly (Drosophila melanogaster)
- B220 antibodyDNA directed RNA polymerase II A antibodyDNA-directed RNA polymerase II largest subunit antibody
- DNA-directed RNA polymerase II largest subunit RNA polymerase II 220 kd subunit antibodyDNA-directed RNA polymerase II subunit A antibodyDNA-directed RNA polymerase II subunit RPB1 antibodyDNA-directed RNA polymerase III largest subunit antibodyhRPB220 antibodyhsRPB1 antibodyPOLR2 antibodyPOLR2A antibodyPOLRA antibodyPolymerase (RNA) II (DNA directed) polypeptide A 220kDa antibodyPolymerase (RNA) II (DNA directed) polypeptide A antibodyRNA polymerase II subunit B1 antibodyRNA-directed RNA polymerase II subunit RPB1 antibodyRPB1 antibodyRPB1_HUMAN antibodyRPB220 antibodyRPBh1 antibodyRpIILS antibodyRPO2 antibodyRPOL2 antibody
Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody [H5] - ChIP Grade images
ab24758 at 1/1000 dilution staining Hela cell by ICC/IF. Cells were paraformaldehyde fixed.
Arabidopsis thaliana root nuclei stained for RNA polymerase II with ab24758 and a polyclonal goat antimouse secondary ab conjugated to FITC (green); Nuclei are counterstained with propidium iodide (PI) - the intensively stained red regions are heterochromatic.
References for Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody [H5] - ChIP Grade (ab24758)
This product has been referenced in:
- Galvão RM et al. Photoactivated phytochromes interact with HEMERA and promote its accumulation to establish photomorphogenesis in Arabidopsis. Genes Dev 26:1851-63 (2012). Read more (PubMed: 22895253) »
- Regnard C et al. Global Analysis of the Relationship between JIL-1 Kinase and Transcription. PLoS Genet 7:e1001327 (2011). ChIP ; Fruit fly (Drosophila melanogaster) . Read more (PubMed: 21423663) »