Anti-RNF25 antibody (ab140514)
Key features and details
- Rabbit polyclonal to RNF25
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RNF25 antibody
See all RNF25 primary antibodies -
Description
Rabbit polyclonal to RNF25 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide, corresponding to a region within amino acids 409-459 of Human RNF25 (NP_071898.2).
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Positive control
- HeLa, 293T and Jurkat whole cell lysate (ab7899)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH: 7-8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab140514 was affinity purified using an epitope specific to RNF25 immobilized on a solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab140514 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 51 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 51 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of NKD2 (By similarity). Stimulates transcription mediated by NF-kappa-B. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 RING-type zinc finger.
Contains 1 RWD domain. -
Domain
The RING-type zinc finger domain interacts with an ubiquitin-conjugating enzyme (E2) and facilitates ubiquitination. -
Post-translational
modificationsUbiquitinated. - Information by UniProt
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Database links
- Entrez Gene: 64320 Human
- SwissProt: Q96BH1 Human
- Unigene: 471403 Human
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Alternative names
- AO7 antibody
- E3 ubiquitin protein ligase RNF25 antibody
- E3 ubiquitin-protein ligase RNF25 antibody
see all
Images
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All lanes : Anti-RNF25 antibody (ab140514) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 51 kDa
Exposure time: 30 seconds -
Immunoprecipitation of RNF25 from HeLa whole cell lysate using ab140514 at 6 µg/mg lysate (1 mg of lysate for IP; 20% of IP loaded).
For blotting immunoprecipitated RNF25, ab140514 was used at 1 µg/ml.
Detection: Chemiluminescence with an exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab140514 has not yet been referenced specifically in any publications.