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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
Concentration information loading...
Purity Immunogen affinity purified
Purification notes ab51475 was affinity purified from monospecific antiserum by immunoaffinity chromatography.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/2000. Detects a band of approximately 54 kDa (predicted molecular weight: 51 kDa).
Use a concentration of 1 µg/ml.
1/10000 - 1/40000.
Function E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of NKD2 (By similarity). Stimulates transcription mediated by NF-kappa-B.
Pathway Protein modification; protein ubiquitination.
Sequence similarities Contains 1 RING-type zinc finger. Contains 1 RWD domain.
Domain The RING-type zinc finger domain interacts with an ubiquitin-conjugating enzyme (E2) and facilitates ubiquitination.
Post-translational modifications Ubiquitinated.
Information by UniProt
E3 ubiquitin protein ligase RNF25 antibody
E3 ubiquitin-protein ligase RNF25 antibody
Anti-RNF25 antibody images
Western blot - RNF25 antibody (ab51475)
All lanes : Anti-RNF25 antibody (ab51475) at 1/1000 dilution Lane 1 : HEK293 lysate from
cells transfected with human
RNF25 Lane 2 : HEK293 lysate from
cells transfected with mouse RNF25 Lane 3 : HEK293 lysate from cells transfected with empty vector Predicted band size : 51 kDa Observed band size : 54 kDa ( why is the actual band size different from the predicted?)
Immunoprecipitation - RNF25 antibody (ab51475)
1ug ab51475 was used in immunoprecipitation of 20 µg HEK293
cell lysate over-expressing HA-tagged human RNF25 (lane 1) or vector only control (lane 2). The precipitated
complex was loaded onto a gel, followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with anti-HA tag antibody.
References for Anti-RNF25 antibody (ab51475)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"