Overview

  • Product name
  • Description
    Rabbit polyclonal to RNF25
  • Tested applications
    Suitable for: WB, IP, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthetic peptide corresponding to amino acids near the carboxyl terminus of human RNF25 protein.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab51475 was affinity purified from monospecific antiserum by immunoaffinity chromatography.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/2000. Detects a band of approximately 54 kDa (predicted molecular weight: 51 kDa).
IP Use a concentration of 1 µg/ml.
ELISA 1/10000 - 1/40000.

Target

  • Function
    E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of NKD2 (By similarity). Stimulates transcription mediated by NF-kappa-B.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 1 RING-type zinc finger.
    Contains 1 RWD domain.
  • Domain
    The RING-type zinc finger domain interacts with an ubiquitin-conjugating enzyme (E2) and facilitates ubiquitination.
  • Post-translational
    modifications
    Ubiquitinated.
  • Information by UniProt
  • Database links
  • Alternative names
    • AO7 antibody
    • E3 ubiquitin protein ligase RNF25 antibody
    • E3 ubiquitin-protein ligase RNF25 antibody
    • EC 6.3.2.- antibody
    • FLJ13906 antibody
    • RING finger protein 25 antibody
    • RING finger protein AO7 antibody
    • RNF 25 antibody
    • RNF25 antibody
    • RNF25_HUMAN antibody
    see all

Images

  • All lanes : Anti-RNF25 antibody (ab51475) at 1/1000 dilution

    Lane 1 : HEK293 lysate from cells transfected with human RNF25
    Lane 2 : HEK293 lysate from cells transfected with mouse RNF25
    Lane 3 : HEK293 lysate from cells transfected with empty vector


    Predicted band size : 51 kDa
    Observed band size : 54 kDa (why is the actual band size different from the predicted?)
  • 1ug ab51475 was used in immunoprecipitation of 20 µg HEK293 cell lysate over-expressing HA-tagged human RNF25 (lane 1) or vector only control (lane 2). The precipitated complex was loaded onto a gel, followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with anti-HA tag antibody.

References

ab51475 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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