Anti-RNF25 antibody (ab51475)

Overview

• Product nameAnti-RNF25 antibodySee all RNF25 primary antibodies ...
• Description
  Rabbit polyclonal to RNF25
• Tested applicationsWB, IP, ELISA more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  A synthetic peptide corresponding to amino acids near the carboxyl terminus of human RNF25 protein.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.01% Sodium Azide
  Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesab51475 was affinity purified from monospecific antiserum by immunoaffinity chromatography.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteasome / Ubiquitin
  • Ubiquitin E2s and E3s
  • RING Finger E3 Ligase

Applications

Target

• FunctionE3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of NKD2 (By similarity). Stimulates transcription mediated by NF-kappa-B.
• PathwayProtein modification; protein ubiquitination.
• Sequence similaritiesContains 1 RING-type zinc finger.
  Contains 1 RWD domain.
• DomainThe RING-type zinc finger domain interacts with an ubiquitin-conjugating enzyme (E2) and facilitates ubiquitination.
• Post-translational
  modificationsUbiquitinated.

Target information above from: UniProt accession Q96BH1 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 64320 Human
  • Entrez Gene: 64320 Human
  • Entrez Gene: 64320 Human
  • Entrez Gene: 57751 Mouse
  • SwissProt: Q96BH1 Human
  • SwissProt: Q96BH1 Human
  • SwissProt: Q96BH1 Human
  • SwissProt: Q9QZR0 Mouse

  • Unigene: 471403 Human
  • Unigene: 280920 Mouse

  see all

• Alternative names
  AO7 antibodyE3 ubiquitin protein ligase RNF25 antibodyE3 ubiquitin-protein ligase RNF25 antibody

  EC 6.3.2.- antibodyFLJ13906 antibodyRING finger protein 25 antibodyRING finger protein AO7 antibodyRNF 25 antibodyRNF25 antibodyRNF25_HUMAN antibody

  see all

Anti-RNF25 antibody images

• 

  Western blot - RNF25 antibody (ab51475)
  All lanes : Anti-RNF25 antibody (ab51475) at 1/1000 dilution
  
  Lane 1 : HEK293 lysate from cells transfected with human RNF25
  Lane 2 : HEK293 lysate from cells transfected with mouse RNF25
  Lane 3 : HEK293 lysate from cells transfected with empty vector
  
  
  Predicted band size : 51 kDa
  Observed band size : 54 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunoprecipitation - RNF25 antibody (ab51475)
  1ug ab51475 was used in immunoprecipitation of 20 µg HEK293 cell lysate over-expressing HA-tagged human RNF25 (lane 1) or vector only control (lane 2). The precipitated complex was loaded onto a gel, followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with anti-HA tag antibody.