Overview

  • Product nameAnti-RNF25 antibodySee all RNF25 primary antibodies ...
  • Description
    Mouse polyclonal to RNF25
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human RNF25 protein (NP_071898.2).

  • Positive control
    • HeLa cell lysate. HeLa nuclear extract. RNF25 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab89281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa.

Target

  • FunctionE3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of NKD2 (By similarity). Stimulates transcription mediated by NF-kappa-B.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 RING-type zinc finger.
    Contains 1 RWD domain.
  • DomainThe RING-type zinc finger domain interacts with an ubiquitin-conjugating enzyme (E2) and facilitates ubiquitination.
  • Post-translational
    modifications
    Ubiquitinated.
  • Information by UniProt
  • Database links
  • Alternative names
    • AO7 antibody
    • E3 ubiquitin protein ligase RNF25 antibody
    • E3 ubiquitin-protein ligase RNF25 antibody
    • EC 6.3.2.- antibody
    • FLJ13906 antibody
    • Ring finger protein 25 antibody
    • RING finger protein AO7 antibody
    • RNF 25 antibody
    • RNF25 antibody
    • RNF25_HUMAN antibody
    see all

Anti-RNF25 antibody images

  • Anti-RNF25 antibody (ab89281) at 1 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size : 51 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • Anti-RNF25 antibody (ab89281) at 1 µg/ml + HeLa Nuclear extract at 50 µg

    Predicted band size : 51 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-RNF25 antibody (ab89281) at 1 µg/ml

    Lane 1 : RNF25 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 51 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa,75 kDa. We are unsure as to the identity of these extra bands.

References for Anti-RNF25 antibody (ab89281)

ab89281 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"