Anti-RNF34 antibody (ab105115)

Overview

• Product nameAnti-RNF34 antibodySee all RNF34 primary antibodies ...
• Description
  Rabbit polyclonal to RNF34
• Tested applicationsWB, IP more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide corresponding to a region within amino acids 200-250 of Human RNF34 (NP_079402.2; GeneID 80196).

• Positive controlHeLa and 293T whole cell lysates

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: Tris citrate/phosphate, pH 7-8
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteasome / Ubiquitin
  • Ubiquitin E2s and E3s
  • RING Finger E3 Ligase

Applications

Target

• FunctionHas E3 ubiquitin-protein ligase activity. Regulates the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. Protects cells against apoptosis induced by TNF. Binds phosphatidylinositol-5-phosphate and phosphatidylinositol-3-phosphate.
• Tissue specificityUbiquitous. Detected in heart, brain, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, colon and leukocytes.
• PathwayProtein modification; protein ubiquitination.
• Sequence similaritiesContains 1 FYVE-type zinc finger.
  Contains 1 RING-type zinc finger.
  Contains 2 SAP domains.
• Post-translational
  modificationsAuto-ubiquitinated (in vitro).
  Proteolytically cleaved by caspases upon induction of apoptosis by TNF.
• Cellular localizationNucleus speckle. Endomembrane system.

Target information above from: UniProt accession Q969K3 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 80196 Human
  • Omim: 608299 Human
  • SwissProt: Q969K3 Human
  • Unigene: 292804 Human

• Alternative names
  CARP 1 antibodyCARP-1 antibodyCARP1 antibody

  Caspase regulator CARP1 antibodyCaspase regulator CARP1 antibodyCaspases-8 and -10-associated RING finger protein 1 antibodyE3 ubiquitin-protein ligase RNF34 antibodyFYVE-RING finger protein Momo antibodyhRFI antibodyHuman RING finger homologous to inhibitor of apoptosis protein antibodyRFI antibodyRIF antibodyRIFF antibodyRING finger protein 34 antibodyRING finger protein RIFF antibodyRNF34 antibodyRNF34_HUMAN antibody

  see all

Anti-RNF34 antibody images

• 

  Western blot - RNF34 antibody (ab105115)
  All lanes : Anti-RNF34 antibody (ab105115) at 0.1 µg/ml
  
  Lane 1 : HeLa whole cell lysate at 50 µg
  Lane 2 : HeLa whole cell lysate at 15 µg
  Lane 3 : HeLa whole cell lysate at 5 µg
  Lane 4 : 293T whole cell lysate at 50 µg
  
  developed using the ECL technique
  
  Predicted band size : 42 kDa
  
  
  Exposure time : 3 minutes
• 

  Immunoprecipitation - RNF34 antibody (ab105115)
  ab105115, at 0.4 µg/ml, staining RNF34 in HeLa whole cell lysate immunoprecipitated using ab105115 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Exposure time: 30 seconds