Overview

  • Product nameAnti-RNF34 antibodySee all RNF34 primary antibodies ...
  • Description
    Rabbit polyclonal to RNF34
  • Tested applicationsWB, IP more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 200-250 of Human RNF34 (NP_079402.2; GeneID 80196).

  • Positive control
    • HeLa and 293T whole cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab105115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 42 kDa.
IP Use a concentration of 2 - 5 µg/ml.

Target

  • FunctionHas E3 ubiquitin-protein ligase activity. Regulates the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. Protects cells against apoptosis induced by TNF. Binds phosphatidylinositol-5-phosphate and phosphatidylinositol-3-phosphate.
  • Tissue specificityUbiquitous. Detected in heart, brain, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, colon and leukocytes.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 FYVE-type zinc finger.
    Contains 1 RING-type zinc finger.
    Contains 2 SAP domains.
  • Post-translational
    modifications
    Auto-ubiquitinated (in vitro).
    Proteolytically cleaved by caspases upon induction of apoptosis by TNF.
  • Cellular localizationNucleus speckle. Endomembrane system.
  • Target information above from: UniProt accession Q969K3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • CARP 1 antibody
    • CARP-1 antibody
    • CARP1 antibody
    • Caspase regulator CARP1 antibody
    • Caspase regulator CARP1 antibody
    • Caspases-8 and -10-associated RING finger protein 1 antibody
    • E3 ubiquitin-protein ligase RNF34 antibody
    • FYVE-RING finger protein Momo antibody
    • hRFI antibody
    • Human RING finger homologous to inhibitor of apoptosis protein antibody
    • RFI antibody
    • RIF antibody
    • RIFF antibody
    • RING finger protein 34 antibody
    • RING finger protein RIFF antibody
    • RNF34 antibody
    • RNF34_HUMAN antibody
    see all

Anti-RNF34 antibody images

  • All lanes : Anti-RNF34 antibody (ab105115) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    developed using the ECL technique

    Predicted band size : 42 kDa


    Exposure time : 3 minutes
  • ab105115, at 0.4 µg/ml, staining RNF34 in HeLa whole cell lysate immunoprecipitated using ab105115 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Exposure time: 30 seconds

References for Anti-RNF34 antibody (ab105115)

ab105115 has not yet been referenced specifically in any publications.

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