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Western blot - RNF8 antibody (ab15850)
(enlarge)
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Product Name
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RNF8 antibody
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See all RNF8 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to RNF8
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Immunogen
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Synthetic peptide: GEPGFFVTGDRAG-C, corresponding to N terminal amino acids 2-14 of Human RNF8.
(Peptide available as ab23278.)
BLAST 'GEPGFFVTGDRAG-C' with  or 
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 0.1 - 1.0 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 56 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human lung lysate.
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Cellular localization
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Nuclear
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> RING Finger E3 Ligase
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Relevance
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RNF8 contains a RING finger motif and a FHA domain. It has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins. Alternatively spliced transcript variants encoding distinct isoforms have been reported. Results suggest a novel function of RNF8 as a regulator of RXR alpha-mediated transcriptional activity through interaction between their respective N-terminal regions.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 0.5mg/ml Tris saline. pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this RNF8 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for RNF8 antibody images, references, products related to ab15850 and other tools.
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RNF8 antibody images:
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 Western blot - RNF8 antibody (ab15850)
Western blot analysis of RNF8
RNF8 antibody (ab15850) at 0.1 µg/ml + Lysates prepared from human lung tissues at 35 µg
Predicted band size : 56 kDa
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References for RNF8 antibody (ab15850)
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Seki N, Hattori A, Sugano S, Suzuki Y, Nakagawara A, Ohhira M, Muramatsu M, Hori T, Saito T. (two most recent references)
Isolation, tissue expression, and chromosomal assignment of a novel human gene which encodes a protein with RING finger motif.
J Hum Genet. 1998;43(4):272-4. PMID: 9852682
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Search PubMed (MEDLINE) for references to RNF8
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RNF8 antibody - more information
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Customer reviews (feedback) regarding RNF8 antibody |
Customer FAQs regarding RNF8 antibody |
Protocols for RNF8 antibody |
Price and availability of products related to RNF8 antibody |
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RNF8 antibody - related products:
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RNF8 antibody - other tools:
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Contact Abcam with a Technical Enquiry about RNF8 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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