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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing
Overview
Product name
Anti-Ribonuclease A antibody
See all Ribonuclease A products (8) ...
Description
Rabbit polyclonal to Ribonuclease A
Tested applications
WB, IP, ELISA, Conjugation, Dot Blot, Flow Cytmore details
Cross reactivity
Reacts with
Cow, Human
Immunogen
Ribonuclease A [Bovine pancreas]
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ribonuclease A [Bovine pancreas].
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing
Applications
Show applications key
Our Abpromise guarantee covers the use of ab6611 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
IP
ELISA
Conjugation
Dot Blot
Flow Cyt
Application notes
Conjugation: Use at an assay dependent concentration.
Dot: Use at an assay dependent concentration.
ELISA: Use at an assay dependent concentration.
Flow Cyt: Use at an assay dependent dilution (See PMID 18812621).
IP: Use at an assay dependent concentration.
WB: Use at an assay dependent concentration.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA.
Tissue specificity
Pancreas and other tissues and body fluids (indicating it may have other physiological functions besides its role in digestion).
Sequence similarities
Belongs to the pancreatic ribonuclease family.
Post-translational
modifications
N-linked glycans are of complex type.
Cellular localization
Secreted.
Target information above from: UniProt accessionP07998
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- HP-RNase antibody
- Pancreatic ribonuclease antibody
- Rib 1 antibody
see all
Database links
- Entrez Gene: 282340 Cow
- Entrez Gene: 6035 Human
- Omim: 180440 Human
- SwissProt: P61823 Cow
- SwissProt: P07998 Human
- SwissProt: Q1KHR2 Human
- Unigene: 78224 Human
References for Anti-Ribonuclease A antibody (ab6611)
This product has been referenced in:
- Fu XM & Zhu BT Both PDI and PDIp can attack the native disulfide bonds in thermally-unfolded RNase and form stable disulfide-linked complexes. Biochim Biophys Acta 1814:487-95 (2011).Read more (PubMed: 21238616) »
- Beerli RRet al. Isolation of human monoclonal antibodies by mammalian cell display. Proc Natl Acad Sci U S A 105:14336-41 (2008). Flow Cyt; Human.Read more (PubMed: 18812621) »
See all 3 publications for this product
Publishing research using ab6611? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"