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Product name
Anti-ROS antibody
See all ROS products (5) ...
Description
Rabbit polyclonal to ROS
Tested applications
ICC/IF, IHC-P, ELISA, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide conjugated to KLH, corresponding to amino acids 39-57 of Human ROS.
Positive control
HL-60 cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
Primary antibody notes
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5512 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml.
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/1000.ELISA: 1/1000.
WB: 1/100 - 1/500. Detects a band of approximately 258 kDa (predicted molecular weight: 281 kDa).
Target
Function
This is probably a cell growth or differentiation factor receptor with a tyrosine-protein kinase activity.
Tissue specificity
Expressed in brain. Expression is increased in primary gliomas.
Involvement in disease
Note=A chromosomal aberration involving ROS1 is found in a glioblastoma multiforme sample. An intra-chromosomal deletion del(6)(q21q21) is responsible for the formation of GOPC-ROS1 chimeric protein which has a constitutive receptor tyrosine kinase activity.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 9 fibronectin type-III domains.
Contains 1 protein kinase domain.
Cellular localization
Membrane.
Target information above from: UniProt accessionP08922
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- c ros 1 antibody
- c ros antibody
- c ros oncogene 1 receptor tyrosine kinase antibody
see all
Database links
- Entrez Gene: 6098 Human
- Omim: 165020 Human
- SwissProt: P08922 Human
- Unigene: 1041 Human
Anti-ROS antibody images:
Western blot - ROS antibody (ab5512)
Predicted band size : 281 kDa
ab5512 at a 1/100 dilution staining approximately 258kDa band of ROS in HL-60 cell lysate by Western blot (ECL).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ROS antibody (ab5512)
ab5512 staining ROS in human hepatocarcinoma (HC) tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Immunocytochemistry/ Immunofluorescence - ROS antibody (ab5512)
ICC/IF image of ab5512 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5512, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-ROS antibody (ab5512)
This product has been referenced in:
- Song SJ et al. Aurora A regulates prometaphase progression by inhibiting the ability of RASSF1A to suppress APC-Cdc20 activity. Cancer Res 69:2314-23 (2009). IHC; Human.Read more (PubMed: 19276349) »
- Jun HJ et al. Epigenetic regulation of c-ROS receptor tyrosine kinase expression in malignant gliomas. Cancer Res 69:2180-4 (2009). IHC-P; Human.Read more (PubMed: 19276365) »
See all 2 publications for this product
Publishing research using ab5512? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"