Recombinant
RabMAb

Anti-RP105 antibody [EPR14720] (ab184956)

Overview

  • Product name
    Anti-RP105 antibody [EPR14720]
    See all RP105 primary antibodies
  • Description
    Rabbit monoclonal [EPR14720] to RP105
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human RP105 aa 150-300. The exact sequence is proprietary.
    Database link: Q99467

  • Positive control
    • Ramos, human lymph node, Daudi lysates, human spleen tissue, Ramos cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab184956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 74 kDa.
IHC-P 1/500.
ICC/IF 1/100.

Target

  • Function
    May cooperate with MD-1 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) in B-cells. Leads to NF-kappa-B activation. Also involved in the life/death decision of B-cells.
  • Tissue specificity
    Expressed mainly on mature peripherical B cells. Detected in spleen, lymph node and appendix. Not detected in pre-B and -T cells.
  • Sequence similarities
    Belongs to the Toll-like receptor family.
    Contains 19 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD180 antibody
    • CD180 antigen antibody
    • CD180_HUMAN antibody
    • F630107B15 antibody
    • LY64 antibody
    • Ly78 antibody
    • Lymphocyte antigen 64 antibody
    • lymphocyte antigen 64 radioprotective 105kDa antibody
    • lymphocyte antigen-64, radioprotective, 105kDa antibody
    • MGC126233 antibody
    • MGC126234 antibody
    • Radioprotective 105 kDa protein antibody
    • RP105 antibody
    see all

Images

  • All lanes : Anti-RP105 antibody [EPR14720] (ab184956) at 1/2000 dilution

    Lane 1 : Human lymph node lysate
    Lane 2 : Ramos cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 74 kDa
    Additional bands at: 90 kDa. We are unsure as to the identity of these extra bands.

  • Anti-RP105 antibody [EPR14720] (ab184956) at 1/2000 dilution + Daudi cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 74 kDa
    Additional bands at: 90 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling RP105 with ab184956 at 1/500 dilution and HRP Polymer to Rabbit IgG.  Counterstained with Hematoxylin.

  • Immunocytochemical analysis of Ramos cells labeling RP105 with ab184956 at 1/100 dilution and Goat anti rabbit IgG (Alexa Fluor®488) at 1/200 dilution. Counterstained with DAPI. Cells fixation: -20℃ Acetone.

References

ab184956 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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