The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/2000.
1/20 - 1/200.
1/1000 - 1/5000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
Function: Acts as a GTPase-activating protein (GAP) involved in trafficking between the Golgi and the ciliary membrane. Involved in localization of proteins, such as NPHP3, to the cilium membrane by inducing hydrolysis of GTP ARL3, leading to the release of UNC119 (or UNC119B). Acts as a GTPase-activating protein (GAP) for tubulin in concert with tubulin-specific chaperone C, but does not enhance tubulin heterodimerization. Acts as guanine nucleotide dissociation inhibitor towards ADP-ribosylation factor-like proteins.
Tissue specificity: Ubiquitous. Expressed in the rod and cone photoreceptors, extending from the tips of the outer segment (OS) through the inner segment (IS) and outer nuclear layer (ONL) and into the synaptic terminals of the outer plexiform layer (ONL). Also detected in the bipolar, horizontal and amacrine cells in the inner nuclear layer (INL), extending to the inner plexiform layer (IPL) and though the ganglion cell layer (GCL) and into the nerve fiber layer (NFL) (at protein level).
Disease: Retinitis pigmentosa 2
Similarity: Belongs to the TBCC family.
Contains 1 C-CAP/cofactor C-like domain.
PTM: Myristoylated on Gly-2; which may be required for membrane targeting.
Palmitoylated on Cys-3; which may be required for plasma membrane targeting (Probable). Mutation of Cys-3 targets the protein to internal membranes.
All lanes : Anti-RP2 antibody (ab229649) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Secondary All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 40 kDa Observed band size: 40 kDa