Anti-RPA32/RPA2 antibody [9H8] (ab2175)

Overview

• Product nameAnti-RPA32/RPA2 antibody [9H8]See all RPA32/RPA2 primary antibodies ...
• Description
  Mouse monoclonal [9H8] to RPA32/RPA2
• SpecificityThis antibody reacts with a 32-34 kDa protein known as Replication Protein A (RPA).
• Tested applicationsFlow Cyt, WB, IP, IHC-P, IHC-Fr, ICC/IF more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Full length protein (Human).

• Positive controlTonsil

Properties

• FormLiquid
• Storage instructionsStore at +4°C.
• Storage bufferPreservative: 0.05% Sodium Azide
  Constituents: 1% BSA
• Concentration information loading...
• PurityIgG fraction
• Clonality Monoclonal
• Clone number9H8
• Myelomaunknown
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • DNA Damage Response
  • DNA Damage Recognition

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Synthesis
  • Other

Applications

Target

• FunctionRequired for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
  Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
• Post-translational
  modificationsPhosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1.
• Cellular localizationNucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.

Target information above from: UniProt accession P15927 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 6118 Human
  • GenBank: NP_002937.1 Human
  • Omim: 179836 Human
  • SwissProt: P15927 Human
  • Unigene: 703070 Human
  • Unigene: 79411 Human

• Alternative names
  60S acidic ribosomal protein P1 antibodyAA409079 antibodyAI325195 antibody

  AU020965 antibodyHSSB antibodyik:tdsubc_2g1 antibodyM(2)21C antibodyMGC137236 antibodyOTTHUMP00000004008 antibodyp32 antibodyp34 antibodyRCJMB04_6d17 replication protein A2, 32kDa antibodyREPA 2 antibodyREPA1 antibodyREPA2 antibodyReplication factor A protein 2 antibodyReplication protein A 32 kDa subunit antibodyReplication protein A 32kDa subunit antibodyReplication protein A 34 kDa subunit antibodyReplication protein A antibodyreplication protein A1 (70kD) antibodyReplication Protein A2 (32kDa) antibodyReplication protein A2 32kD antibodyReplication protein A2 32kDa antibodyReplication protein A2 antibodyReplication protein A2, 32kDa antibodyRF A antibodyRF-A protein 2 antibodyRf-A2 antibodyRFA antibodyRFA2_HUMAN antibodyRP A antibodyRP-A p32 antibodyRP-A p34 antibodyRP21C antibodyRPA 2 antibodyRPA 32 antibodyRPA antibodyRPA2 antibodyRPA32 antibodyRPA34 antibodyRPA70 antibodyRpLP1 antibodyRpP2 antibodyxx:tdsubc_2g1 antibodyzgc:109822 antibody

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Anti-RPA32/RPA2 antibody [9H8] images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RPA32/RPA2 antibody [9H8] (ab2175)
  ab2175 - immunohistochemistryFormalin fixed paraffin embedded human tonsil stained with RPA using ABC and AEC chromogen.
• 

  Immunocytochemistry/ Immunofluorescence - RPA32/RPA2 antibody [9H8] (ab2175)This image is courtesy of an Abreview submitted by Dr Kok-Lung Chan

  ab2175 at 1/200 staining human skin fibroblasts by ICC/IF. The cells were treated with 2mM hydroxyurea for 16 hours, formaldehyde fixed and permeabilized with 0.5% Triton X100. They were then incubated with the primary antibody for 1.5 hours at 37°C. A Cy3 ® conjugated sheep anti-mouse antibody was used as the secondary.

  See Abreview

• 

  Western blot - RPA32/RPA2 antibody [9H8] (ab2175)
  All lanes : Anti-RPA32/RPA2 antibody [9H8] (ab2175) at 5 µg/ml
  
  Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
  Lane 2 : SK N BE (Human neuroblastoma) Whole Cell Lysate
  Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Predicted band size : 32 kDa
  Observed band size : 32 kDa
  
• 

  Flow Cytometry - Anti-RPA32/RPA2 antibody [9H8] (ab2175)

  Anti-RPA/32/RPA2 antibody [9H8] (ab2175) is used to measure DNA Double-Stranded Breaks resection in U2OS cells +/- Camptotechin (CPT).

  ab2175 is used at 1/200 dilution in 1xPBS + 0.2% Triton X-100 for 1 hour at 25°C.

  Secondary antibody: anti-mouse Molecular Probes Alexa Fluor® 488 Conjugated at 1/200 dilution.

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [9H8] (ab2175)
  Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue, staining RPA32/RPA2 with ab2175. Staining was detected using DAB.
• 

  Flow Cytometry - Anti-RPA32/RPA2 antibody [9H8] (ab2175)
  Overlay histogram showing HeLa cells stained with ab2175 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2175, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.