Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1.
Nucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
RCJMB04_6d17 replication protein A2, 32kDa antibody
Replication factor A protein 2 antibody
Replication protein A 32 kDa subunit antibody
Replication protein A 32kDa subunit antibody
Replication protein A 34 kDa subunit antibody
Replication protein A antibody
Replication Protein A2 (32kDa) antibody
Replication protein A2 antibody
Replication protein A2, 32kDa antibody
RF-A protein 2 antibody
RP-A p32 antibody
RP-A p34 antibody
RPA 2 antibody
RPA 32 antibody
Western blot - Anti-RPA32/RPA2 (phospho T21) antibody - N-terminal (ab229459)
All lanes : Anti-RPA32/RPA2 (phospho T21) antibody - N-terminal (ab229459) at 1/1000 dilution
Lane 1 : HEK293T (human epithelial cell line from embryonic kidney) whole cell extract Lane 2 : HEK293T (human epithelial cell line from embryonic kidney) whole cell extract treated with 100 µM Etoposide for 2 hours
Lysates/proteins at 30 µg per lane.
Predicted band size: 29 kDa
12% SDS-PAGE gel.
has not yet been referenced specifically in any publications.
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