Anti-RPA32/RPA2 (phospho T21) antibody - N-terminal (ab229459)


  • Product name
    Anti-RPA32/RPA2 (phospho T21) antibody - N-terminal
    See all RPA32/RPA2 primary antibodies
  • Description
    Rabbit polyclonal to RPA32/RPA2 (phospho T21) - N-terminal
  • Host species
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig, Zebrafish, Rhesus monkey
  • Immunogen

    Synthetic peptide within Human RPA32/RPA2 (N terminal) (phospho T21). The exact sequence is proprietary. Conjugated to a protein carrier.
    Database link: P15927

  • Positive control
    • WB: HEK-293T whole cell extract.



Our Abpromise guarantee covers the use of ab229459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 29 kDa.


  • Function
    Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
    Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
  • Post-translational
    Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1.
  • Cellular localization
    Nucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
  • Information by UniProt
  • Database links
  • Alternative names
    • 60S acidic ribosomal protein P1 antibody
    • AA409079 antibody
    • AI325195 antibody
    • AU020965 antibody
    • ik:tdsubc_2g1 antibody
    • M(2)21C antibody
    • MGC137236 antibody
    • OTTHUMP00000004008 antibody
    • p32 antibody
    • p34 antibody
    • RCJMB04_6d17 replication protein A2, 32kDa antibody
    • REPA2 antibody
    • Replication factor A protein 2 antibody
    • Replication protein A 32 kDa subunit antibody
    • Replication protein A 32kDa subunit antibody
    • Replication protein A 34 kDa subunit antibody
    • Replication protein A antibody
    • Replication Protein A2 (32kDa) antibody
    • Replication protein A2 antibody
    • Replication protein A2, 32kDa antibody
    • RF-A protein 2 antibody
    • Rf-A2 antibody
    • RFA antibody
    • RFA2_HUMAN antibody
    • RP-A p32 antibody
    • RP-A p34 antibody
    • RP21C antibody
    • RPA 2 antibody
    • RPA 32 antibody
    • RPA antibody
    • Rpa2 antibody
    • RPA32 antibody
    • RPA34 antibody
    • RpLP1 antibody
    • RpP2 antibody
    • xx:tdsubc_2g1 antibody
    • zgc:109822 antibody
    see all


  • All lanes : Anti-RPA32/RPA2 (phospho T21) antibody - N-terminal (ab229459) at 1/1000 dilution

    Lane 1 : HEK293T (human epithelial cell line from embryonic kidney) whole cell extract
    Lane 2 : HEK293T (human epithelial cell line from embryonic kidney) whole cell extract treated with 100 µM Etoposide for 2 hours

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 29 kDa

    12% SDS-PAGE gel.


ab229459 has not yet been referenced specifically in any publications.

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