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Overview

  • Product nameRPL5 protein (Tagged-His Tag)See all RPL5 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 99-297 (199 amino acids) of Human RPL5 with an N terminal His tag. Predicted mwt: 24 kDa; UniProt ID: P46777.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag.
  • FormLyophilised:Reconstitute with 46 µl distilled water.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Sequence notesYCTGLLLARRLLNRFGMDKIYEGQVEVTGDEYNVESIDGQ PGAFTCYLDAGLA RTTTGNKVFGALKGAVDGGLSIPHS TKRFPGYDSESKEFNAEVHRKHIMGQNV ADYMRYLMEE DEDAYKKQFSQYIKNSVTPDMMEEMYKKAHAAIRENPVYE KKP KKEVKKKRWNRPKMSLAQKKDRVAQKKASFLRAQE RAAES
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91956 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        60S ribosomal protein L5MSTP030Ribosomal protein L5
        RL5RL5_HUMANRP L5RPL 5RPL5U21 RNAU21RNA
      see all
  • FunctionRequired for rRNA maturation and formation of the 60S ribosomal subunits. This protein binds 5S RNA.
  • Involvement in diseaseDefects in RPL5 are the cause of Diamond-Blackfan anemia type 6 (DBA6) [MIM:612561]. DBA6 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies.
  • Sequence similaritiesBelongs to the ribosomal protein L18P family.
  • Cellular localizationCytoplasm. Nucleus > nucleolus.

    • Target information above from: UniProt accession P46777 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    RPL5 protein (Tagged-His Tag) images

    • SDS-PAGE - RPL5 protein (Tagged-His Tag) (ab91956)
      SDS-PAGE - RPL5 protein (Tagged-His Tag) (ab91956)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for RPL5 protein (Tagged-His Tag) (ab91956)

    ab91956 has not yet been referenced specifically in any publications.

    Publishing research using ab91956 ? Please let us know so that we can cite the reference in this datasheet

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