Anti-RPLP1 antibody (ab121190)
Key features and details
- Rabbit polyclonal to RPLP1
- Suitable for: ICC, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RPLP1 antibody
See all RPLP1 primary antibodies -
Description
Rabbit polyclonal to RPLP1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to amino acids 1-109 of Human RPLP1 (P05386).
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Positive control
- WB: HeLa whole cell lysate; IHC-P: Human cerebral cortex, cerebellum, skin, and prostate tissues; ICC: U-2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121190 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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Notes |
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ICC
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
Target
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Function
Plays an important role in the elongation step of protein synthesis. -
Sequence similarities
Belongs to the ribosomal protein L12P family. - Information by UniProt
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Database links
- Entrez Gene: 6176 Human
- Omim: 180520 Human
- SwissProt: P05386 Human
- Unigene: 356502 Human
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Alternative names
- 60S acidic ribosomal protein P1 antibody
- RLA1_HUMAN antibody
- Rplp1 antibody
Images
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All lanes : Anti-RPLP1 antibody (ab121190) at 0.4 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) transfected with control siRNA, whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) transfected with RPLP1 specific siRNA probe #1, whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) transfected with RPLP1 specific siRNA probe #2, whole cell lysateRemaining relative intensity is presented.
Loading control: Anti-GAPDH.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue labelling RPLP1 with ab121190 at 1/200 dilution. Heat mediated antigen retrieval with citrate buffer pH 6 performed before commencing with IHC staining protocol.
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Immunocytochemistry analysis of U-2 OS cells labelling RPLP1 with ab121190 at 2 µg/mL. Cells were fixed with 4% paraformaldehyde and permeabilized with Triton X-100.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue labelling RPLP1 with ab121190 at 1/200 dilution. Heat mediated antigen retrieval with citrate buffer pH 6 performed before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebellum tissue labelling RPLP1 with ab121190 at 1/200 dilution. Heat mediated antigen retrieval with citrate buffer pH 6 performed before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling RPLP1 with ab121190 at 1/200 dilution. Heat mediated antigen retrieval with citrate buffer pH 6 performed before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab121190 has been referenced in 3 publications.
- Campos RK et al. Ribosomal stalk proteins RPLP1 and RPLP2 promote biogenesis of flaviviral and cellular multi-pass transmembrane proteins. Nucleic Acids Res 48:9872-9885 (2020). PubMed: 32890404
- He Z et al. RPLP1 promotes tumor metastasis and is associated with a poor prognosis in triple-negative breast cancer patients. Cancer Cell Int 18:170 (2018). PubMed: 30386179
- Campos RK et al. RPLP1 and RPLP2 Are Essential Flavivirus Host Factors That Promote Early Viral Protein Accumulation. J Virol 91:N/A (2017). PubMed: 27974556