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Anti-RPS19 antibody (ab40833) at 0.03 µg/ml + Human K562 lysate in RIPA buffer at 35 µg
Predicted band size : 16 kDa
Observed band size : 15 kDa (why is the actual band size different from the predicted?)
Anti-RPS19 antibody (ab40833) at 0.1 µg/ml + lysate prepared from transfected HEK293 cells, transiently expressing RPS19
Predicted band size : 16 kDa
Observed band size : 18 kDa (why is the actual band size different from the predicted?)
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