Feedback Form

Western blot - RPS19 antibody (ab40833)
Western blot - RPS19 antibody (ab40833)
(enlarge)


Product Name 

RPS19 antibody

 

See all RPS19 antibodies (2)...

Product type 

Primary antibodies

Description 

Goat polyclonal to RPS19

Immunogen 

Synthetic peptide: with C-KMVEKDQDGGRK sequence from the internal region of Human RPS19.
BLAST 'KMVEKDQDGGRK' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 

Hu

Predicted to react 
(species key)

Ms, Rat, Cat, Chmp, Cow, Dog, RMk
(Due to sequence homology)

Tested applications 
(see key)

ELISA, WB


Abreviews 

Customer reviews
(see key)


Read about Abreviews
(Read all Abreviews)

Submit an Abreview and earn up to 220 Abpoints by reviewing this product

• WB

1 Abreview

- average rating: 2 stars (out of 5)2 stars (out of 5)2 stars (out of 5)2 stars (out of 5)2 stars (out of 5)


WB
(read Abreview)


Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution. (Antibody detection limit dilution 1:64,000).
WB: Use at a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 16 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Daudi and K562 cell lysates.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome

Relevance 

RPS19 belongs to the ribosomal protein S19e family. Defects in this gene are a primary cause of Diamond-Blackfan anemia (DBA). DBA is a hypoplastic macrocytic anemia characterized by absent or decreased levels of erythroid precursors in bone marrow, which usually appears in the first year of life.

 

Alternative names RPS19 antibody (ab40833)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

282051    (Cow)
6223    (Human)

GenBank

    

NP_001013.1    (Human)

Omim

    

603474    (Human)

SwissProt

    

Q32PD5    (Cow)
P39019    (Human)
Q9CZX8    (Mouse)
P17074    (Rat)

Unigene

    

438429    (Human)

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this RPS19 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for RPS19 antibody images, references, products related to ab40833 and other tools.

 

RPS19 antibody images:

Product image  Western blot - RPS19 antibody (ab40833)

Western blot - RPS19 antibody (ab40833)

RPS19 antibody (ab40833) at 0.03 µg/ml + Human K562 lysate in RIPA buffer at 35 µg

Predicted band size : 16 kDa
Observed band size : 15 kDa (why is the actual band size different from the predicted?)

 
 

Search PubMed (MEDLINE) for references to RPS19

 

RPS19 antibody - more information

Customer reviews (feedback) regarding RPS19 antibody

Customer FAQs regarding RPS19 antibody

Protocols for RPS19 antibody

Price and availability of products related to RPS19 antibody

 

RPS19 antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Positive Controls for RPS19 antibody

ab3951

  

Daudi (Human Burkitt's lymphoma cell line) Whole Cell Lysate

ab7911

  

K-562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate

 

Compatible Secondaries for RPS19 antibody

ab6566

  

Cy5 ® antibody

ab6739

  

Texas Red ® antibody

ab6740

  

Biotin antibody

ab6741

  

HRP antibody

ab6742

  

Alkaline Phosphatase antibody

ab6881

  

FITC antibody

ab7004

  

Phycoerythrin antibody

ab27245

  

10nm Gold antibody

ab27246

  

10nm Gold antibody

ab27247

  

15nm Gold antibody

 

Other RPS19 Antibodies

See all RPS19 antibodies (2)...

 

RPS19 antibody - other tools:

Contact Abcam with a Technical Enquiry about RPS19 antibody

 

All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

©1998-2010 Abcam plc. All rights reserved.