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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome
Overview
Product name
Anti-RPS19 antibody
See all RPS19 products (3) ...
Description
Goat polyclonal to RPS19
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Cat, Dog, Chimpanzee, Rhesus monkey
Immunogen
Synthetic peptide: with C-KMVEKDQDGGRK sequence from the internal region of Human RPS19.
KMVEKDQDGG RK
Positive control
Daudi and K562 cell lysates. Lysate prepared from transfected HEK293 transiently expressing RPS19.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome
Applications
Show applications key
Our Abpromise guarantee covers the use of ab40833 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent concentration. (PubMed: 20206555)
WB: Use a concentration of 0.03 - 0.1 µg/ml.Detects a band of approximately 15 kDa (predicted molecular weight: 16 kDa).
Target
Function
Required for pre-rRNA processing and maturation of 40S ribosomal subunits.
Tissue specificity
Higher level expression is seen in the colon carcinoma tissue than normal colon tissue.
Involvement in disease
Defects in RPS19 are the cause of Diamond-Blackfan anemia type 1 (DBA1) [MIM:105650]. DBA1 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies.
Sequence similarities
Belongs to the ribosomal protein S19e family.
Cellular localization
Nucleus. Located more specifically in the nucleoli.
Target information above from: UniProt accessionP39019
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 40S ribosomal protein S19 antibody
- DBA antibody
- DBA1 antibody
see all
Database links
- Entrez Gene: 282051 Cow
- Entrez Gene: 6223 Human
- Entrez Gene: 100503302 Mouse
- Entrez Gene: 20085 Mouse
- Entrez Gene: 29287 Rat
- GenBank: NP_001013.1 Human
- Omim: 603474 Human
- SwissProt: Q32PD5 Cow
see all
Anti-RPS19 antibody images:
Western blot - RPS19 antibody (ab40833)
Anti-RPS19 antibody (ab40833) at 0.03 µg/ml + Human K562 lysate in RIPA buffer at 35 µg
Predicted band size : 16 kDa
Observed band size : 15 kDa (why is the actual band size different from the predicted?)
Western blot - RPS19 antibody (ab40833)
Anti-RPS19 antibody (ab40833) at 0.1 µg/ml + lysate prepared from transfected HEK293 cells, transiently expressing RPS19
Predicted band size : 16 kDa
Observed band size : 18 kDa (why is the actual band size different from the predicted?)
References for Anti-RPS19 antibody (ab40833)
This product has been referenced in:
- Horos Ret al. Ribosomal deficiencies in Diamond-Blackfan anemia impair translation of transcripts essential for differentiation of murine and human erythroblasts. Blood 119:262-72 (2012). WB; Mouse.Read more (PubMed: 22058113) »
- Ponpuak Met al. Delivery of cytosolic components by autophagic adaptor protein p62 endows autophagosomes with unique antimicrobial properties. Immunity 32:329-41 (2010). ICC/IF; Mouse.Read more (PubMed: 20206555) »
See all 2 publications for this product
Publishing research using ab40833? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"