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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome
Overview
Product name
Anti-RPS19 antibody
See all RPS19 products (3) ...
Description
Mouse monoclonal to RPS19
Tested applications
WB, Flow Cytmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein, corresponding to amino acids 1-146 of Human RPS19
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG2b
Light chain type
kappa
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome
Applications
Show applications key
Our Abpromise guarantee covers the use of ab57643 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Predicted molecular weight: 16 kDa.
Flow Cyt: Use 1µg for 106 cells.
Target
Function
Required for pre-rRNA processing and maturation of 40S ribosomal subunits.
Tissue specificity
Higher level expression is seen in the colon carcinoma tissue than normal colon tissue.
Involvement in disease
Defects in RPS19 are the cause of Diamond-Blackfan anemia type 1 (DBA1) [MIM:105650]. DBA1 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies.
Sequence similarities
Belongs to the ribosomal protein S19e family.
Cellular localization
Nucleus. Located more specifically in the nucleoli.
Target information above from: UniProt accessionP39019
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 40S ribosomal protein S19 antibody
- DBA antibody
- DBA1 antibody
see all
Database links
- Entrez Gene: 6223 Human
- GenBank: NP_001013.1 Human
- Omim: 603474 Human
- SwissProt: P39019 Human
- Unigene: 438429 Human
Anti-RPS19 antibody images:
Western blot - RPS19 antibody (ab57643)
RPS19 antibody (ab57643) at 1ug/lane + K-562 cell lysate at 25ug/lane.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS19 antibody (ab57643)
IHC-P image of RPS19 antibody (ab57643) on Kidney sections. The sections were fixed in Paraformaldehyde and underwent heat mediated antigen retrieval using Novacastra (pH6). The sections were then blocked in 0.3% H2O2 solution for 10 minutes at 22°C, before addition of the primary antibody at 1/100 dilution for 2 hours.
Aamir Ahmed, University College London, United Kingdom.
Flow Cytometry - Anti-RPS19 antibody (ab57643)
Overlay histogram showing HCT116 cells stained with ab57643 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57643, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HCT116 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
References for Anti-RPS19 antibody (ab57643)
This product has been referenced in:
- Bykhovskaya Yet al. Phenotypic expression of maternally inherited deafness is affected by RNA modification and cytoplasmic ribosomal proteins. Mol Genet Metab 97:297-304 (2009).Read more (PubMed: 19482502) »
See 1 publication for this product
Publishing research using ab57643? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"