Anti-RPS27A antibody (ab57646)
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
PBS, pH 7.2
- Concentration information loading...
- PurityProtein G purified
- Clonality Monoclonal
- Light chain typelambda
- Research Areas
Our Abpromise guarantee covers the use of ab57646 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: Use a concentration of 1 - 5 µg/ml.|
|Flow Cyt||Flow Cyt: Use 0.1µg for 106 cells.|
- RelevanceRPS27A (Ribosomal protein S27a) is a component of the 40S subunit of the ribosome. It is synthesized as a C-terminal extension protein (CEP) of ubiquitin. The fusion protein consists of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. The protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a.
- 40S ribosomal protein S27a antibody
- CEP 80 antibody
- CEP80 antibody
- HUBCEP 80 antibody
- HUBCEP80 antibody
- Ribosomal protein S27a antibody
- RPS 27A antibody
- UBA 80 antibody
- UBA80 antibody
- UBCEP 1 antibody
- UBCEP 80 antibody
- UBCEP1 antibody
- UBCEP80 antibody
- Ubiquitin and ribosomal protein S27a antibody
- Ubiquitin carboxyl extension protein 80 antibody
- Ubiquitin CEP80 antibody
Anti-RPS27A antibody images
Overlay histogram showing HeLa cells stained with ab57646 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57646, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
RPS27A antibody (ab57646) at 1ug/lane + HL-60 cell lysate at 25ug/lane.
References for Anti-RPS27A antibody (ab57646)
ab57646 has not yet been referenced specifically in any publications.