Overview

  • Product nameAnti-RPS27A antibody
    See all RPS27A primary antibodies
  • Description
    Mouse monoclonal to RPS27A
  • Tested applicationsWB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-157 of Human RPS27A

Properties

Applications

Our Abpromise guarantee covers the use of ab57646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml.
Flow Cyt Use 0.1µg for 106 cells.

Target

  • RelevanceRPS27A (Ribosomal protein S27a) is a component of the 40S subunit of the ribosome. It is synthesized as a C-terminal extension protein (CEP) of ubiquitin. The fusion protein consists of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. The protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a.
  • Database links
  • Alternative names
    • 40S ribosomal protein S27a antibody
    • CEP 80 antibody
    • CEP80 antibody
    • HUBCEP 80 antibody
    • HUBCEP80 antibody
    • Ribosomal protein S27a antibody
    • RPS 27A antibody
    • UBA 80 antibody
    • UBA80 antibody
    • UBCEP 1 antibody
    • UBCEP 80 antibody
    • UBCEP1 antibody
    • UBCEP80 antibody
    • Ubiquitin and ribosomal protein S27a antibody
    • Ubiquitin carboxyl extension protein 80 antibody
    • Ubiquitin CEP80 antibody
    see all

Anti-RPS27A antibody images

  • Overlay histogram showing HeLa cells stained with ab57646 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57646, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was an anti-mouse Alexa Fluor® 488 (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • RPS27A antibody (ab57646) at 1ug/lane + HL-60 cell lysate at 25ug/lane.

References for Anti-RPS27A antibody (ab57646)

ab57646 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"