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Overview

  • Product nameRPS27A protein (Tagged-His Tag)
  • Protein descriptionRecombinant full length protein, corresponding to amino acids 1-156 of Human RPS27A with N terminal His tag; Predicted MWt 19 kDa (UniProt Q5RKT7).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 101 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Sequence notesMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQ QRLIFAGKQ LEDGRTLSDYNIQKESTLHLVLRLRGGAK KRKKKSYTTPKKNKHKRKKV KLAVLKYYKVDENGKISR LRRECPSDECGAGVFMASHFDRHYCGKCCLT YCFNKPE DK
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab92095 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        40S ribosomal protein S27aCEP 80CEP80
        HUBCEP 80HUBCEP80Ribosomal protein S27aRPS 27AUBA 80UBA80UBCEP 1UBCEP 80UBCEP1UBCEP80Ubiquitin and ribosomal protein S27aUbiquitin carboxyl extension protein 80Ubiquitin CEP80
      see all
  • RelevanceRPS27A (Ribosomal protein S27a) is a component of the 40S subunit of the ribosome. It is synthesized as a C-terminal extension protein (CEP) of ubiquitin. The fusion protein consists of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. The protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a.

    • RPS27A protein (Tagged-His Tag) images

    • SDS-PAGE - RPS27A protein (Tagged-His Tag) (ab92095)
      SDS-PAGE - RPS27A protein (Tagged-His Tag) (ab92095)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for RPS27A protein (Tagged-His Tag) (ab92095)

    ab92095 has not yet been referenced specifically in any publications.

    Publishing research using ab92095 ? Please let us know so that we can cite the reference in this datasheet

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