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Anti-RRAS antibody (ab47536)

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Overview

Product name

Anti-RRAS antibody
See all RRAS products (4) ...

Description

Rabbit polyclonal to RRAS

Tested applications

IHC-P, WB, IPmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide: RGRPRGGGPGPRDPPPGETHK, corresponding to internal sequence amino acids 11-31 of Human RRAS. The peptide immunogen sequence is 100% conserved in rat and mouse.

RGRPRGGGPG PRDPPPGETH K

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Heart, colon, and spleen tissue, CA cell lines.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: Whole serum

Purity

Whole antiserum

Clonality

Polyclonal

Isotype

IgG

Research areas

Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins

  • Western blot - RRAS antibody (ab47536)Western blot - RRAS antibody (ab47536) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab47536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • IHC-P

     IHC-P: 1/1000 - 1/5000.

    IHC-P: 1/1000 - 1/5000.

  • ShowHide1 Image

    WB

     WB: 1/1000 - 1/2000.Pred...Read more →

    WB: 1/1000 - 1/2000.Predicted molecular weight: 24 kDa.

  • IP

     IP: 1/50 - 1/200.

    IP: 1/50 - 1/200.

Target

Function

Regulates the organization of the actin cytoskeleton.

Sequence similarities

Belongs to the small GTPase superfamily. Ras family.

Cellular localization

Cell membrane. Inner surface of plasma membrane possibly with attachment requiring acylation of the C-terminal cysteine.

Target information above from: UniProt accessionP10301 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Oncogene RRAS antibody
  • p23 antibody
  • R Ras antibody
  • Ras related Protein antibody
  • Ras related protein R Ras antibody
  • Ras-related protein R-Ras antibody
  • related RAS viral (r ras) oncogene homolog antibody
  • RRAS antibody
  • RRAS_HUMAN antibody
see all

Database links

see all

Anti-RRAS antibody images:

  Western blot - RRAS antibody (ab47536)

Western blot - RRAS antibody (ab47536)

Anti-RRAS antibody (ab47536) at 1/500 dilution + PC3 (Human prostate carcinoma cell line) Whole Cell Lysate Whole Cell Lysate at 10 µg

Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 24 kDa
Observed band size : 26 kDa (why is the actual band size different from the predicted?)


Exposure time : 1 minute

References for Anti-RRAS antibody (ab47536)

ab47536 has not yet been referenced specifically in any publications.

Publishing research using ab47536? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"