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Read our guarantee »Products:Signal Transduction >> Metabolism >> Energy Metabolism
Anti-RSF1 antibody
See all RSF1 products (3) ...
Mouse polyclonal to RSF1
Reacts with
Human
Predicted to work with
Mouse, Cow, Dog, Chimpanzee, Macaque Monkey
Recombinant fragment: RLHRIETDEE ESCDNAHGDA NQPARDSQPR VLPSEQESTK KPYRIESDEE EDFENVGKVG SPLDYSLVDL PSTNGQSPGK AIENLIGKPT EKSQTPKDNS , corresponding to amino acids 1047-1146 of Human RSF1
RLHRIETDEE ESCDNAHGDA NQPARDSQPR VLPSEQESTK KPYRIESDEE EDFENVGKVG SPLDYSLVDL PSTNGQSPGK AIENLIGKPT EKSQTPKDNS
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: None
Constituents: 50% Glycerol, Whole serum
Whole antiserum
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
Polyclonal
IgG
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing
Signal Transduction >> Metabolism >> Energy Metabolism
Western blot - RSF1 antibody (ab43632)
(enlarge)
Our Abpromise guarantee covers the use of ab43632 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000. Predicted molecular weight: 163 kDa. This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RSF1 is involved in transcription repression when recruited to DNA via the DNA binding of GAL4. It is also involved in chromatin remodeling and spacing when associated with SNF2H and transcription coactivation when associated with hepatitis B virus X protein (HBX). The RSF1 gene is localized to the long arm of human chromosome 11 between q13.4 and q14.1. This region is amplified and rearranged in many tumors, indicating that it plays a role in tumorigenesis similar to that of other PHD-containing proteins.
Nuclear
Western blot - RSF1 antibody (ab43632)

All lanes : Anti-RSF1 antibody (ab43632) at 1/1000 dilution
Lane 1 : ~20ug of a total protein extract from E coli with 50ng of a tagged protein of an irrelevant antigen
Lane 2 : ~20ug of a total protein extract from E coli with 50ng of the antigen (tagged fusion protein)
Secondary
Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution
Predicted band size : 163 kDa
This product has been referenced in:
See 1 publication for this product
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All lanes : Anti-RSF1 antibody (ab43632) at 1/1000 dilution
Lane 1 : ~20ug of a total protein extract from E coli with 50ng of a tagged protein of an irrelevant antigen
Lane 2 : ~20ug of a total protein extract from E coli with 50ng of the antigen (tagged fusion protein)
Secondary
Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution
Predicted band size : 163 kDa
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