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Anti-RSF1 antibody (ab43632)

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Overview

Product name

Anti-RSF1 antibody
See all RSF1 products (3) ...

Description

Mouse polyclonal to RSF1

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Cow, Dog, Chimpanzee, Macaque Monkey

Immunogen

Recombinant fragment: RLHRIETDEE ESCDNAHGDA NQPARDSQPR VLPSEQESTK KPYRIESDEE EDFENVGKVG SPLDYSLVDL PSTNGQSPGK AIENLIGKPT EKSQTPKDNS , corresponding to amino acids 1047-1146 of Human RSF1

RLHRIETDEE ESCDNAHGDA NQPARDSQPR VLPSEQESTK KPYRIESDEE EDFENVGKVG SPLDYSLVDL PSTNGQSPGK AIENLIGKPT EKSQTPKDNS

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 50% Glycerol, Whole serum

Purity

Whole antiserum

Primary antibody notes

This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Clonality

Polyclonal

Isotype

IgG

  • Western blot - RSF1 antibody (ab43632)Western blot - RSF1 antibody (ab43632) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab43632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

WB: 1/1000. Predicted molecular weight: 163 kDa. This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

RSF1 is involved in transcription repression when recruited to DNA via the DNA binding of GAL4. It is also involved in chromatin remodeling and spacing when associated with SNF2H and transcription coactivation when associated with hepatitis B virus X protein (HBX). The RSF1 gene is localized to the long arm of human chromosome 11 between q13.4 and q14.1. This region is amplified and rearranged in many tumors, indicating that it plays a role in tumorigenesis similar to that of other PHD-containing proteins.

Cellular localization

Nuclear

Alternative names

  • HXBAP antibody
  • p325 antibody
  • remodeling and spacing factor 1 antibody
  • RSF 1 antibody
  • XAP8 antibody
see all

Anti-RSF1 antibody images:

  Western blot - RSF1 antibody (ab43632)

Western blot - RSF1 antibody (ab43632)

All lanes : Anti-RSF1 antibody (ab43632) at 1/1000 dilution

Lane 1 : ~20ug of a total protein extract from E coli with 50ng of a tagged protein of an irrelevant antigen
Lane 2 : ~20ug of a total protein extract from E coli with 50ng of the antigen (tagged fusion protein)

Secondary
Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

Predicted band size : 163 kDa

References for Anti-RSF1 antibody (ab43632)

This product has been referenced in:

  • Goldman JAet al. Chromatin remodeling by imitation switch (ISWI) class ATP-dependent remodelers is stimulated by histone variant H2A.Z. J Biol Chem 285:4645-51 (2010). WB; Human.Read more (PubMed: 19940112) »

See 1 publication for this product

Publishing research using ab43632? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"