RSF1 antibody (ab43632)

Product Name 

RSF1 antibody

Product type 

Primary antibodies

Description 

Mouse polyclonal to RSF1

Immunogen 

Recombinant fragment: RLHRIETDEE ESCDNAHGDA NQPARDSQPR VLPSEQESTK KPYRIESDEE EDFENVGKVG SPLDYSLVDL PSTNGQSPGK AIENLIGKPT EKSQTPKDNS , corresponding to amino acids 1047-1146 of Human RSF1
BLAST 'RLHRIETDEE ESCDNAHGDA NQPARDSQPR VLPSEQESTK KPYRIESDEE EDFENVGKVG SPLDYSLVDL PSTNGQSPGK AIENLIGKPT EKSQTPKDNS ' with or

Reacts with 

Hu

Predicted to react 
(species key)

Ms, Chmp, Cow, Dog, Mcq
(Due to sequence homology)

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/1000. Predicted molecular weight: 163 kDa. This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing
Signal Transduction >> Metabolism >> Energy Metabolism

Relevance 

RSF1 is involved in transcription repression when recruited to DNA via the DNA binding of GAL4. It is also involved in chromatin remodeling and spacing when associated with SNF2H and transcription coactivation when associated with hepatitis B virus X protein (HBX).
The RSF1 gene is localized to the long arm of human chromosome 11 between q13.4 and q14.1. This region is amplified and rearranged in many tumors, indicating that it plays a role in tumorigenesis similar to that of other PHD-containing proteins.

This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Database links 

Raised in 

Mouse

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Preservative: None
Constituents: 50% Glycerol, Whole serum

Material safety datasheet (MSDS) for this product:
Glycerol MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

See below for RSF1 antibody images, references, products related to ab43632 and other tools.

RSF1 antibody images:

Customer reviews (feedback) regarding RSF1 antibody

Customer FAQs regarding RSF1 antibody

Protocols for RSF1 antibody

Price and availability of products related to RSF1 antibody

RSF1 antibody - related products: