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Anti-RSF1 antibody (ab83322)

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Overview

Product name

Anti-RSF1 antibody
See all RSF1 products (3) ...

Description

Rabbit polyclonal to RSF1

Tested applications

ELISA, WBmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Rat, Pig

Immunogen

Synthetic peptide corresponding to a region within internal sequence amino acids 1116-1165 (QDEFVVSDEN PDESEEDPPS NDDSDTDFCS RRLRRHPSRP MRQSRRLRRK) of human RSF1 (NP_057662).

QDEFVVSDEN PDESEEDPPS NDDSDTDFCS RRLRRHPSRP MRQSRRLRRK

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Human fetal lung lysate

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 2% Sucrose, PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

Purified by peptide affinity chromatography method.

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing
Signal Transduction >> Metabolism >> Energy Metabolism

  • Western blot - RSF1 antibody (ab83322)Western blot - RSF1 antibody (ab83322) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab83322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ELISA: Use at an assay dependent dilution.
ELISA titre using peptide based assay, 1:12500
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 163 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

RSF1 is involved in transcription repression when recruited to DNA via the DNA binding of GAL4. It is also involved in chromatin remodeling and spacing when associated with SNF2H and transcription coactivation when associated with hepatitis B virus X protein (HBX). The RSF1 gene is localized to the long arm of human chromosome 11 between q13.4 and q14.1. This region is amplified and rearranged in many tumors, indicating that it plays a role in tumorigenesis similar to that of other PHD-containing proteins.

Cellular localization

Nuclear

Alternative names

  • HXBAP antibody
  • p325 antibody
  • remodeling and spacing factor 1 antibody
  • RSF 1 antibody
  • XAP8 antibody
see all

Database links

Anti-RSF1 antibody images:

  Western blot - RSF1 antibody (ab83322)

Western blot - RSF1 antibody (ab83322)

Anti-RSF1 antibody (ab83322) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal lung lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 163 kDa
Observed band size : 168 kDa (why is the actual band size different from the predicted?)


Gel Concentration: 6-18%

References for Anti-RSF1 antibody (ab83322)

ab83322 has not yet been referenced specifically in any publications.

Publishing research using ab83322? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"