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Western blot - RSF1 antibody (ab83322)
(enlarge)
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Product Name
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RSF1 antibody
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See all RSF1 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to RSF1
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Immunogen
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Synthetic peptide corresponding to a region within internal sequence amino acids 1116-1165 (QDEFVVSDEN PDESEEDPPS NDDSDTDFCS RRLRRHPSRP MRQSRRLRRK) of human RSF1 (NP_057662).
BLAST 'QDEFVVSDEN PDESEEDPPS NDDSDTDFCS RRLRRHPSRP MRQSRRLRRK ' with  or 
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
ELISA titre using peptide based assay, 1:12500
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 163 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human fetal lung lysate
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing
Signal Transduction >> Metabolism >> Energy Metabolism
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Relevance
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RSF1 is involved in transcription repression when recruited to DNA via the DNA binding of GAL4. It is also involved in chromatin remodeling and spacing when associated with SNF2H and transcription coactivation when associated with hepatitis B virus X protein (HBX).
The RSF1 gene is localized to the long arm of human chromosome 11 between q13.4 and q14.1. This region is amplified and rearranged in many tumors, indicating that it plays a role in tumorigenesis similar to that of other PHD-containing proteins.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Purification notes
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Purified by peptide affinity chromatography method.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this RSF1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for RSF1 antibody images, references, products related to ab83322 and other tools.
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RSF1 antibody images:
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 Western blot - RSF1 antibody (ab83322)
RSF1 antibody (ab83322) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal lung lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 163 kDa
Observed band size : 168 kDa (why is the actual band size different from the predicted?)
Gel Concentration: 6-18%
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Search PubMed (MEDLINE) for references to RSF1
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RSF1 antibody - more information
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Customer reviews (feedback) regarding RSF1 antibody |
Customer FAQs regarding RSF1 antibody |
Protocols for RSF1 antibody |
Price and availability of products related to RSF1 antibody |
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RSF1 antibody - related products:
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RSF1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about RSF1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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