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Anti-RSK1 p90 antibody [E4] (ab32114)

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Overview

Product name

Anti-RSK1 p90 antibody [E4]
See all RSK1 p90 products (25) ...

Description

Rabbit monoclonal [E4] to RSK1 p90

Tested applications

WB, IHC-P, ICC, Flow Cyt, IPmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

A synthetic peptide corresonding to residues in N terminus of human RSK1 p90.

Positive control

WB: MCF7 cell lysate. IHC-P: Human colon tissue.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Concentration

Concentration information loading...

Clonality

Monoclonal

Clone number

E4

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> MAPK Pathway

  • Western blot - RSK1 p90 antibody [E4] (ab32114)Western blot - RSK1 p90 antibody [E4] (ab32114) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - RSK1 p90 antibody [E4] (ab32114)Immunohistochemistry (Paraffin-embedded sections) - RSK1 p90 antibody [E4] (ab32114) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab32114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

    Four stars (1 Abreview) WB: 1/1000. Detects a ba...Read more →

    WB: 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa).

  • 1 Image

    IHC-P

     IHC-P: 1/100.

    IHC-P: 1/100.

  • ICC

     ICC: 1/200.

    ICC: 1/200.

  • Flow Cyt

     Flow Cyt: 1/50.

    Flow Cyt: 1/50.

  • IP

     IP: 1/10.

    IP: 1/10.

Target

Function

Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains.

Post-translational
modifications

Autophosphorylated on Ser-380, as part of the activation process.

Target information above from: UniProt accessionQ15418 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 90 kDa ribosomal protein S6 kinase 1 antibody
  • dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody
  • dJ590P13.1 antibody
  • EC 2.7.11.1 antibody
  • HU 1 antibody
  • HU1 antibody
  • KS6A1_HUMAN antibody
  • MAP kinase activated protein kinase 1a antibody
  • MAP kinase-activated protein kinase 1a antibody
  • MAPK-activated protein kinase 1a antibody
  • MAPKAP kinase 1a antibody
  • MAPKAPK-1a antibody
  • MAPKAPK1A antibody
  • MGC79981 antibody
  • Mitogen-activated protein kinase-activated protein kinase 1A antibody
  • OTTHUMP00000004113 antibody
  • p90 RSK1 antibody
  • p90-RSK 1 antibody
  • p90rsk antibody
  • p90RSK1 antibody
  • p90S6K antibody
  • pp90RSK1 antibody
  • Ribosomal protein S6 kinase 90kD 1 antibody
  • Ribosomal protein S6 kinase 90kD polypeptide 1 antibody
  • Ribosomal protein S6 kinase 90kDa polypeptide 1 antibody
  • Ribosomal protein S6 kinase alpha 1 antibody
  • Ribosomal protein S6 kinase alpha-1 antibody
  • Ribosomal protein S6 kinase polypeptide 1 antibody
  • Ribosomal S6 kinase 1 antibody
  • RPS6K1 alpha antibody
  • rps6ka antibody
  • Rps6ka1 antibody
  • RSK 1 antibody
  • RSK 1 p90 antibody
  • RSK antibody
  • RSK-1 antibody
  • RSK1 antibody
  • S6K alpha 1 antibody
  • S6K-alpha-1 antibody
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Database links

see all

Anti-RSK1 p90 antibody [E4] images:

  Western blot - RSK1 p90 antibody [E4] (ab32114)

Western blot - RSK1 p90 antibody [E4] (ab32114)

Anti-RSK1 p90 antibody [E4] (ab32114) at 1/1000 dilution + MCF7 cell lysate

Predicted band size : 83 kDa
Observed band size : 90 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry (Paraffin-embedded sections) - RSK1 p90 antibody [E4] (ab32114)

Immunohistochemistry (Paraffin-embedded sections) - RSK1 p90 antibody [E4] (ab32114)

Ab32114, at a 1/100 dilution, staining RSK1 p90 in paraffin embedded human colon tissue by immunohistochemistry.

References for Anti-RSK1 p90 antibody [E4] (ab32114)

This product has been referenced in:

  • McPhee JB  et al. Delineation of regions of the Yersinia YopM protein required for interaction with the RSK1 and PRK2 host kinases and their requirement for interleukin-10 production and virulence. Infect Immun 78:3529-39 (2010). WB.Read more (PubMed: 20515922) »

See 1 publication for this product

Publishing research using ab32114? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"