![Immunocytochemistry/ Immunofluorescence - RSK1 p90 (phospho S380) antibody [E239] (ab32203) image](#ab32203if.jpg)
![Western blot - RSK1 p90 (phospho S380) antibody [E239] (ab32203) image](#ab32203wb copy.jpg)
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Product name
Anti-RSK1 p90 (phospho S380) antibody [E239]
See all RSK1 p90 products (25) ...
Description
Rabbit monoclonal [E239] to RSK1 p90 (phospho S380)
Specificity
The antibody only detects RSK1 phosphorylated on Serine 380.
Tested applications
WB, ICC/IF, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Ser380 of human p90RSK1.
Positive control
A431 cell lysates
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
E239
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> MAPK Pathway
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32203 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: 1/250 - 1/500.
IP: 1/80.
WB: 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa).
Is unsuitable for FACS or IHC.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains.
Post-translational
modifications
Autophosphorylated on Ser-380, as part of the activation process.
Target information above from: UniProt accessionQ15418
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 90 kDa ribosomal protein S6 kinase 1 antibody
- dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody
- dJ590P13.1 antibody
see all
Database links
- Entrez Gene: 6195 Human
- Entrez Gene: 20111 Mouse
- Entrez Gene: 81771 Rat
- Omim: 601684 Human
- SwissProt: Q15418 Human
- SwissProt: P18653 Mouse
- SwissProt: Q63531 Rat
- Unigene: 149957 Human
see all
Anti-RSK1 p90 (phospho S380) antibody [E239] images:
Immunocytochemistry/ Immunofluorescence - RSK1 p90 (phospho S380) antibody [E239] (ab32203)
![Immunocytochemistry/ Immunofluorescence - RSK1 p90 (phospho S380) antibody [E239] (ab32203)](/ps/datasheet/Images/32/ab32203/ab32203if.jpg)
Immunofluorescent staining of A431 cells using ab32203 at 1/250 dilution.
Western blot - RSK1 p90 (phospho S380) antibody [E239] (ab32203)
![Western blot - RSK1 p90 (phospho S380) antibody [E239] (ab32203)](/ps/datasheet/Images/32/ab32203/ab32203wb copy.jpg)
All lanes : Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203) at 1/10000 dilution
Lane 1 : A431 cell lysate, untreated
Lane 2 : A431 cell lysate, treated with EGF
Predicted band size : 83 kDa
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
References for Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203)
This product has been referenced in:
- Hamdi MM & Mutungi G Dihydrotestosterone stimulates amino acid uptake and the expression of LAT2 in mouse skeletal muscle fibres through an ERK1/2-dependent mechanism. J Physiol 589:3623-40 (2011). WB; Mouse.Read more (PubMed: 21606113) »
See 1 publication for this product
Publishing research using ab32203? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"