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Anti-RSK1 p90 (phospho T359 + S363) antibody [E238] (ab32413)

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Overview

Product name

Anti-RSK1 p90 (phospho T359 + S363) antibody [E238]
See all RSK1 p90 products (25) ...

Description

Rabbit monoclonal [E238] to RSK1 p90 (phospho T359 + S363)

Specificity

This antibody is specific for human RSK1 p90 phoshorylated on Threonine 359 and Serine 363.

Tested applications

WB, IHC-P, ICC, IPmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic phospho peptide corresponding to residues surrounding Threonine 359 and Serine 363 of Human RSK1 p90.

Positive control

WB: A431 cell lysate. IHC-P: Human cervical carcinoma tissue.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Concentration

Concentration information loading...

Clonality

Monoclonal

Clone number

E238

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> MAPK Pathway

  • Western blot - RSK1 p90 (phospho T359 + S363) antibody [E238] (ab32413)Western blot - RSK1 p90 (phospho T359 + S363) antibody [E238] (ab32413) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RSK1 p90 (phospho T359 + S363) antibody [E238] (ab32413)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RSK1 p90 (phospho T359 + S363) antibody [E238] (ab32413) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab32413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC: 1/50.
IHC-P: 1/100.
IP: 1/40.
WB: 1/20000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).

Is unsuitable for FACS.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains.

Post-translational
modifications

Autophosphorylated on Ser-380, as part of the activation process.

Target information above from: UniProt accessionQ15418 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 90 kDa ribosomal protein S6 kinase 1 antibody
  • dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody
  • dJ590P13.1 antibody
  • EC 2.7.11.1 antibody
  • HU 1 antibody
  • HU1 antibody
  • KS6A1_HUMAN antibody
  • MAP kinase activated protein kinase 1a antibody
  • MAP kinase-activated protein kinase 1a antibody
  • MAPK-activated protein kinase 1a antibody
  • MAPKAP kinase 1a antibody
  • MAPKAPK-1a antibody
  • MAPKAPK1A antibody
  • MGC79981 antibody
  • Mitogen-activated protein kinase-activated protein kinase 1A antibody
  • OTTHUMP00000004113 antibody
  • p90 RSK1 antibody
  • p90-RSK 1 antibody
  • p90rsk antibody
  • p90RSK1 antibody
  • p90S6K antibody
  • pp90RSK1 antibody
  • Ribosomal protein S6 kinase 90kD 1 antibody
  • Ribosomal protein S6 kinase 90kD polypeptide 1 antibody
  • Ribosomal protein S6 kinase 90kDa polypeptide 1 antibody
  • Ribosomal protein S6 kinase alpha 1 antibody
  • Ribosomal protein S6 kinase alpha-1 antibody
  • Ribosomal protein S6 kinase polypeptide 1 antibody
  • Ribosomal S6 kinase 1 antibody
  • RPS6K1 alpha antibody
  • rps6ka antibody
  • Rps6ka1 antibody
  • RSK 1 antibody
  • RSK 1 p90 antibody
  • RSK antibody
  • RSK-1 antibody
  • RSK1 antibody
  • S6K alpha 1 antibody
  • S6K-alpha-1 antibody
see all

Database links

see all

Anti-RSK1 p90 (phospho T359 + S363) antibody [E238] images:

  Western blot - RSK1 p90 (phospho T359 + S363) antibody [E238] (ab32413)

Western blot - RSK1 p90 (phospho T359 + S363) antibody [E238] (ab32413)

All lanes : Anti-RSK1 p90 (phospho T359 + S363) antibody [E238] (ab32413) at 1/20000 dilution

Lane 1 : A431 cell lysate untreated.
Lane 2 : A431 cell lysate treated with EGF.


Predicted band size : 90 kDa
Observed band size : 90 kDa

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RSK1 p90 (phospho T359 + S363) antibody [E238] (ab32413)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RSK1 p90 (phospho T359 + S363) antibody [E238] (ab32413)

Ab32413, at a 1/100 dilution, staining RSK1 p90 in paraffin embedded human cervical carcinoma tissue by Immunohistochemistry.
Left image : Untreated.
Right image : Treated with Phosphatase.

References for Anti-RSK1 p90 (phospho T359 + S363) antibody [E238] (ab32413)

This product has been referenced in:

  • Chia KM  et al. A Feedback Loop between Androgen Receptor and ERK Signaling in Estrogen Receptor-Negative Breast Cancer. Neoplasia 13:154-66 (2011). IHC-P.Read more (PubMed: 21403841) »

See 1 publication for this product

Publishing research using ab32413? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"