Anti-RTF1 antibody (ab99362)
Key features and details
- Rabbit polyclonal to RTF1
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RTF1 antibody
See all RTF1 primary antibodies -
Description
Rabbit polyclonal to RTF1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Tilapia, Orangutan, Xenopus tropicalis, Medaka fish -
Immunogen
Immunogen corresponding to C terminal amino acids within Human RTF1 (EAW92492.1).
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Positive control
- HeLa whole cell lysate (ab150035).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab99362 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 77 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 77 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Plays a role in transcription-coupled histone modification. Plays a role in regulation of transcription. Required for maximal induction of heat-shock genes (By similarity). Binds single-stranded DNA. -
Sequence similarities
Contains 1 Plus3 domain. -
Domain
The Plus3 domain mediates single-stranded DNA-binding. -
Cellular localization
Nucleus > nucleoplasm. - Information by UniProt
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Database links
- Entrez Gene: 423215 Chicken
- Entrez Gene: 23168 Human
- Entrez Gene: 76246 Mouse
- Entrez Gene: 366169 Rat
- Omim: 611633 Human
- SwissProt: Q92541 Human
- SwissProt: A2AQ19 Mouse
- SwissProt: Q08EC5 Mouse
see all -
Alternative names
- CDG1N antibody
- GTL 7 antibody
- GTL7 antibody
see all
Images
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All lanes : Anti-RTF1 antibody (ab99362) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 77 kDa
Exposure time: 10 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin carcinoma (left) and mouse teratoma (right) tissues labelling RTF1 with ab99362 at 1/1000 (1µg/ml). Detection: DAB.
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Immunoprecipitation : 1 mg of HeLa cell lysate immunoprecipitated with ab99362 at 3 µg/mg lysate; 20% of immunoprecipitate loaded in lane
Western Blot: RTF1 antibody (ab99362) at 1 µg/ml
developed using the ECL technique
Exposure time : 3 seconds
Predicted band size : 77 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab99362 has been referenced in 1 publication.
- Eirin A et al. Comparative proteomic analysis of extracellular vesicles isolated from porcine adipose tissue-derived mesenchymal stem/stromal cells. Sci Rep 6:36120 (2016). PubMed: 27786293