Anti-RTKN/Rhotekin antibody (ab154954)
Key features and details
- Rabbit polyclonal to RTKN/Rhotekin
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RTKN/Rhotekin antibody
See all RTKN/Rhotekin primary antibodies -
Description
Rabbit polyclonal to RTKN/Rhotekin -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Recombinant fragment corresponding to Human RTKN/Rhotekin aa 1-141.
Database link: Q9BST9 -
Positive control
- WB: 293T, A431, Jurkat, Raji and mouse brain whole cell lysates. A431 cells. ICC/IF: Rhotekin-transfected HeLa cells.
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General notes
Keep as concentrated solution.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154954 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 63 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 63 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Mediates Rho signaling to activate NF-kappa-B and may confer increased resistance to apoptosis to cells in gastric tumorigenesis. May play a novel role in the organization of septin structures. -
Tissue specificity
Highly expressed in prostate, moderately in kidney, heart, brain, spleen, testis, placenta, small intestine, pancreas, skeletal muscle and peripheral blood leukocytes, and weakly in ovary, colon and thymus. Weakly expressed in all normal cell lines tested. Overexpressed in various cancer cell lines. -
Sequence similarities
Contains 1 PH domain.
Contains 1 REM (Hr1) repeat. - Information by UniProt
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Database links
- Entrez Gene: 6242 Human
- Entrez Gene: 20166 Mouse
- Entrez Gene: 297383 Rat
- Omim: 602288 Human
- SwissProt: Q9BST9 Human
- SwissProt: Q8C6B2 Mouse
- SwissProt: Q6V7V2 Rat
- Unigene: 192854 Human
see all -
Alternative names
- Rhotekin antibody
- Rtkn antibody
- RTKN_HUMAN antibody
- RTKN1 antibody
Images
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All lanes : Anti-RTKN/Rhotekin antibody (ab154954) at 1/5000 dilution
Lane 1 : Jurkat whole cell lysate
Lane 2 : Raji whole cell lysate
Lane 3 : NCI-H929 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 63 kDa -
Rhotekin-transfected (right) or untransfected (left) HeLa cells were fixed in ice-cold MeOH for 5 min. Stained with ab154954 diluted at 1/500 (green). Nuclear staining using Hoechst 33342 (blue).
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All lanes : Anti-RTKN/Rhotekin antibody (ab154954) at 1/500 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : human Rhotekin-transfected 293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 63 kDa -
Anti-RTKN/Rhotekin antibody (ab154954) at 1/1000 dilution + Jurkat whole cell lysate at 30 µg
Predicted band size: 63 kDa
7.5% SDS PAGE -
Anti-RTKN/Rhotekin antibody (ab154954) at 1/10000 dilution + mouse brain whole cell lysate at 20 µg
Predicted band size: 63 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde-fixed A431 cells labeling RTKN/Rhotekin with ab154954 at 1/200 dilution (green). The image in the lower panel is costained with Hoechst 33342 (blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab154954 has been referenced in 2 publications.
- Zhou J et al. MicroRNA-152 inhibits tumor cell growth by directly targeting RTKN in hepatocellular carcinoma. Oncol Rep 37:1227-1234 (2017). PubMed: 27922690
- Zhang W et al. Inhibition of rhotekin exhibits antitumor effects in lung cancer cells. Oncol Rep 35:2529-34 (2016). WB . PubMed: 26935528