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Western blot - Runx1/AML1 antibody (ab23980)
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ChIP - RUNX1 / AML1 antibody - ChIP Grade (ab23980)
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ChIP - RUNX1 / AML1 antibody - ChIP Grade (ab23980)
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Product Name
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RUNX1 / AML1 antibody - ChIP Grade
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See all RUNX1 / AML1 antibodies (14)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to RUNX1 / AML1 - ChIP Grade
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human Runx1/AML1.
(Peptide available as ab24287.)
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Reacts with
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Hu, Ms
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Predicted to react
(species key)
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Rat
(Due to sequence homology)
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Tested applications
(see key)
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ChIP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ChIP: Use at an assay dependent dilution (See Abreview).
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 48 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Jurkat Nuclear Lysate
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Cellular localization
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Nuclear
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Research areas
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Developmental Biology >> Organogenesis >> Hematopoietic system development
Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Stem Cells >> Hematopoietic Progenitors >> Hemangioblast
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Transcription factors
Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Stem Cells >> Hematopoietic Progenitors >> Intracellular Molecules
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Neuroscience >> Neurology process >> Neurogenesis
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Relevance
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AML1/Runx1 binds DNA as a monomer and through the Runt domain. DNA binding is increased by heterodimerization with CBFB. Isoform AML1L can neither bind DNA nor heterodimerize and interferes with the transactivation activity of AML1/Runx1. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T cell receptor enhancers, LCK, IL3 and GMCSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. AML1/Runx1 is expressed in a wide variety of tissues and is expressed at the highest levels in thymus, bone marrow and peripheral blood. Defects in AML1/Runx1 are the cause of familial platelet disorder with associated myeloid malignancy, an autosomal dominant disease characterized by qualitative and quantitative platelet defects, and propensity to develop acute myelogenous leukemia.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.80 - 0.80 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this RUNX1 / AML1 antibody - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for RUNX1 / AML1 antibody - ChIP Grade images, references, products related to ab23980 and other tools.
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RUNX1 / AML1 antibody - ChIP Grade images:
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 ChIP - RUNX1 / AML1 antibody - ChIP Grade (ab23980)
Chromatin was prepared from nuclear lysate of the mouse haematopoietic progenitor cell line HPC-7. The cross-linking (X-ChiP) technique was used, crosslinking was performed for 10 minutes in 1% formaldehyde. The primary antibody was used undiluted and incubated with the sample for 16 hours at 4°C. The immunoprecipitated DNA was quantified by real time PCR. This image is a courtesy of Anonimous Abreview
See Abreview
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ChIP - RUNX1 / AML1 antibody - ChIP Grade (ab23980)
ab23980 used undiluted in ChIP analysis, incubated for 16 hours at 4°C.Nuclear cell lysate prepared from mouse haematopoietic progenitor cell line HPC-7.Cross-linking (X-ChIP) performed by using 1% formaldehyde for 10 minutes.Detection step: Real time PCR.Negative control: A regultory element which is an open region of chromatin but is devoid of this transcription factor. This image is courtesy of an anonymous abreview.
See Abreview
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References for RUNX1 / AML1 antibody - ChIP Grade (ab23980)
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This product has been used in:
Gardini A et al. AML1/ETO oncoprotein is directed to AML1 binding regions and co-localizes with AML1 and HEB on its targets. PLoS Genet 4:e1000275 (2008). PubMed: 19043539
If you publish research using ab23980 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to RUNX1 / AML1
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RUNX1 / AML1 antibody - ChIP Grade - more information
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Customer reviews (feedback) regarding RUNX1 / AML1 antibody - ChIP Grade |
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Price and availability of products related to RUNX1 / AML1 antibody - ChIP Grade |
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RUNX1 / AML1 antibody - ChIP Grade - related products:
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RUNX1 / AML1 antibody - ChIP Grade - other tools:
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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