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Product Name
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RUNX1 / AML1 antibody
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See all RUNX1 / AML1 antibodies (14)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to RUNX1 / AML1
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Immunogen
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Synthetic peptide: PHHPAPTPNPRAS conjugated to BSA, corresponding to C terminal amino acids 213-225 of Human RUNX1 / AML1
BLAST 'PHHPAPTPNPRAS' with  or 
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Reacts with
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Hu, Ms, Rat
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Predicted to react
(species key)
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Glla
(Due to sequence homology)
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Tested applications
(see key)
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GSA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
GSA: Use at an assay dependent dilution.
WB: 1/200 - 1/1000. Predicted molecular weight: 49 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Developmental Biology >> Organogenesis >> Hematopoietic system development
Stem Cells >> Hematopoietic Progenitors >> Hemangioblast
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Transcription factors
Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Stem Cells >> Hematopoietic Progenitors >> Intracellular Molecules
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Neuroscience >> Neurology process >> Neurogenesis
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Relevance
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AML1/Runx1 binds DNA as a monomer and through the Runt domain. DNA binding is increased by heterodimerization with CBFB. Isoform AML1L can neither bind DNA nor heterodimerize and interferes with the transactivation activity of AML1/Runx1. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T cell receptor enhancers, LCK, IL3 and GMCSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. AML1/Runx1 is expressed in a wide variety of tissues and is expressed at the highest levels in thymus, bone marrow and peripheral blood. Defects in AML1/Runx1 are the cause of familial platelet disorder with associated myeloid malignancy, an autosomal dominant disease characterized by qualitative and quantitative platelet defects, and propensity to develop acute myelogenous leukemia.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 50% Ammonium sulphate, PBS
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Form
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Liquid
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this RUNX1 / AML1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to RUNX1 / AML1
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RUNX1 / AML1 antibody - more information
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Customer reviews (feedback) regarding RUNX1 / AML1 antibody |
Customer FAQs regarding RUNX1 / AML1 antibody |
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RUNX1 / AML1 antibody - related products:
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RUNX1 / AML1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about RUNX1 / AML1 antibody |
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