Anti-RUNX1 / AML1 antibody (ab54869)

Overview

• Product nameAnti-RUNX1 / AML1 antibodySee all RUNX1 / AML1 primary antibodies ...
• Description
  Mouse monoclonal to RUNX1 / AML1
• Tested applicationsWB, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant fragment, corresponding to amino acids 210-311 of Human RUNX1 / AML1

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  PBS, pH 7.2
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• IsotypeIgG2b
• Light chain typekappa
• Research Areas
  • Developmental Biology
  • Organogenesis
  • Hematopoietic system development

  • Stem Cells
  • Hematopoietic Progenitors
  • Hemangioblast

  • Stem Cells
  • Hematopoietic Progenitors
  • Intracellular Molecules

  • Cancer
  • Oncoproteins/suppressors
  • Oncoproteins
  • Transcription factors

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

  • Epigenetics and Nuclear Signaling
  • Chromatin Binding Proteins
  • DNA / RNA binding

  • Neuroscience
  • Neurology process
  • Neurogenesis

Applications

Target

• FunctionCBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits MYST4-dependent transcriptional activation.
• Tissue specificityExpressed in all tissues examined except brain and heart. Highest levels in thymus, bone marrow and peripheral blood.
• Involvement in diseaseNote=A chromosomal aberration involving RUNX1/AML1 is a cause of M2 type acute myeloid leukemia (AML-M2). Translocation t(8;21)(q22;q22) with RUNX1T1.
  Note=A chromosomal aberration involving RUNX1/AML1 is a cause of therapy-related myelodysplastic syndrome (T-MDS). Translocation t(3;21)(q26;q22) with EAP or MECOM.
  Note=A chromosomal aberration involving RUNX1/AML1 is a cause of chronic myelogenous leukemia (CML). Translocation t(3;21)(q26;q22) with EAP or MECOM.
  Note=A chromosomal aberration involving RUNX1/AML1 is found in childhood acute lymphoblastic leukemia (ALL). Translocation t(12;21)(p13;q22) with TEL. The translocation fuses the 3'-end of TEL to the alternate 5'-exon of AML-1H.
  Note=A chromosomal aberration involving RUNX1 is found in acute leukemia. Translocation t(11,21)(q13;q22) that forms a MACROD1-RUNX1 fusion protein.
  Defects in RUNX1 are the cause of familial platelet disorder with associated myeloid malignancy (FPDMM) [MIM:601399]. FPDMM is an autosomal dominant disease characterized by qualitative and quantitative platelet defects, and propensity to develop acute myelogenous leukemia.
  Note=A chromosomal aberration involving RUNX1/AML1 is found in therapy-related myeloid malignancies. Translocation t(16;21)(q24;q22) that forms a RUNX1-CBFA2T3 fusion protein.
  Note=A chromosomal aberration involving RUNX1/AML1 is a cause of chronic myelomonocytic leukemia. Inversion inv(21)(q21;q22) with USP16.
• Sequence similaritiesContains 1 Runt domain.
• DomainA proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes.
• Post-translational
  modificationsPhosphorylated in its C-terminus upon IL-6 treatment. Phosphorylation enhances interaction with MYST3.
  Methylated.
• Cellular localizationNucleus.

Target information above from: UniProt accession Q01196 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 861 Human
  • Omim: 151385 Human
  • SwissProt: Q01196 Human
  • Unigene: 149261 Human
  • Unigene: 612648 Human

• Alternative names
  Acute myeloid leukemia 1 antibodyAcute myeloid leukemia 1 protein antibodyalpha subunit core binding factor antibody

  AML 1 antibodyAML1 antibodyAML1 EVI 1 antibodyAML1 EVI 1 fusion protein antibodyAml1 oncogene antibodyAMLCR 1 antibodyAMLCR1 antibodyCBF alpha 2 antibodyCBF-alpha-2 antibodyCBFA 2 antibodyCBFA2 antibodyCore binding factor alpha 2 subunit antibodyCore binding factor runt domain alpha subunit 2 antibodyCore-binding factor subunit alpha-2 antibodyEVI 1 antibodyEVI1 antibodyHGNC antibodyOncogene AML 1 antibodyOncogene AML-1 antibodyOTTHUMP00000108696 antibodyOTTHUMP00000108697 antibodyOTTHUMP00000108699 antibodyOTTHUMP00000108700 antibodyOTTHUMP00000108702 antibodyPEA2 alpha B antibodyPEA2-alpha B antibodyPEBP2 alpha B antibodyPEBP2-alpha B antibodyPEBP2A2 antibodyPEBP2aB antibodyPolyomavirus enhancer binding protein 2 alpha B subunit antibodyPolyomavirus enhancer-binding protein 2 alpha B subunit antibodyRun1 antibodyRunt related transcription factor 1 antibodyRunt-related transcription factor 1 antibodyRUNX 1 antibodyRunx1 antibodyRUNX1_HUMAN antibodySL3 3 enhancer factor 1 alpha B subunit antibodySL3-3 enhancer factor 1 alpha B subunit antibodySL3/AKV core binding factor alpha B subunit antibodySL3/AKV core-binding factor alpha B subunit antibody

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Anti-RUNX1 / AML1 antibody images

• 

  IHC-P - RUNX1 / AML1 antibody (ab54869)
  RUNX1 / AML1 antibody (ab54869) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human placenta.
• 

  Western blot - RUNX1 / AML1 antibody (ab54869)
  
  
  Predicted band size : 49 kDa
  RUNX1 / AML1 antibody (ab54869) at 1ug/lane + HeLa cell lysate at 25ug/lane.
• 

  Immunocytochemistry/ Immunofluorescence-RUNX1 / AML1 antibody(ab54869)
  ab54869 at 10 ug/ml staining RUNX1 / AML1 in human Hela cells by Immunocytochemistry / Immunofluorescence.