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Anti-RUNX1 / AML1 antibody (ab55998)

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Overview

  • Product nameAnti-RUNX1 / AML1 antibodySee all RUNX1 / AML1 primary antibodies ...
  • Description
    Rabbit polyclonal to RUNX1 / AML1
  • Tested applicationsWB, ELISA more details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to N-terminal residues of human RUNX1 / AML1.

Properties

Applications

Our Abpromise guarantee covers the use of ab55998 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 49 kDa. for 2 hours. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA ELISA: Use at an assay dependent dilution.

Target

  • FunctionCBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits MYST4-dependent transcriptional activation.
  • Tissue specificityExpressed in all tissues examined except brain and heart. Highest levels in thymus, bone marrow and peripheral blood.
  • Involvement in diseaseNote=A chromosomal aberration involving RUNX1/AML1 is a cause of M2 type acute myeloid leukemia (AML-M2). Translocation t(8;21)(q22;q22) with RUNX1T1.
    Note=A chromosomal aberration involving RUNX1/AML1 is a cause of therapy-related myelodysplastic syndrome (T-MDS). Translocation t(3;21)(q26;q22) with EAP or MECOM.
    Note=A chromosomal aberration involving RUNX1/AML1 is a cause of chronic myelogenous leukemia (CML). Translocation t(3;21)(q26;q22) with EAP or MECOM.
    Note=A chromosomal aberration involving RUNX1/AML1 is found in childhood acute lymphoblastic leukemia (ALL). Translocation t(12;21)(p13;q22) with TEL. The translocation fuses the 3'-end of TEL to the alternate 5'-exon of AML-1H.
    Note=A chromosomal aberration involving RUNX1 is found in acute leukemia. Translocation t(11,21)(q13;q22) that forms a MACROD1-RUNX1 fusion protein.
    Defects in RUNX1 are the cause of familial platelet disorder with associated myeloid malignancy (FPDMM) [MIM:601399]. FPDMM is an autosomal dominant disease characterized by qualitative and quantitative platelet defects, and propensity to develop acute myelogenous leukemia.
    Note=A chromosomal aberration involving RUNX1/AML1 is found in therapy-related myeloid malignancies. Translocation t(16;21)(q24;q22) that forms a RUNX1-CBFA2T3 fusion protein.
    Note=A chromosomal aberration involving RUNX1/AML1 is a cause of chronic myelomonocytic leukemia. Inversion inv(21)(q21;q22) with USP16.
  • Sequence similaritiesContains 1 Runt domain.
  • DomainA proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes.
  • Post-translational
    modifications    Phosphorylated in its C-terminus upon IL-6 treatment. Phosphorylation enhances interaction with MYST3.
    Methylated.
  • Cellular localizationNucleus.

    • Target information above from: UniProt accession Q01196 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      Acute myeloid leukemia 1 antibodyAcute myeloid leukemia 1 protein antibodyalpha subunit core binding factor antibody
      AML 1 antibodyAML1 antibodyAML1 EVI 1 antibodyAML1 EVI 1 fusion protein antibodyAml1 oncogene antibodyAMLCR 1 antibodyAMLCR1 antibodyCBF alpha 2 antibodyCBF-alpha-2 antibodyCBFA 2 antibodyCBFA2 antibodyCore binding factor alpha 2 subunit antibodyCore binding factor runt domain alpha subunit 2 antibodyCore-binding factor subunit alpha-2 antibodyEVI 1 antibodyEVI1 antibodyHGNC antibodyOncogene AML 1 antibodyOncogene AML-1 antibodyOTTHUMP00000108696 antibodyOTTHUMP00000108697 antibodyOTTHUMP00000108699 antibodyOTTHUMP00000108700 antibodyOTTHUMP00000108702 antibodyPEA2 alpha B antibodyPEA2-alpha B antibodyPEBP2 alpha B antibodyPEBP2-alpha B antibodyPEBP2A2 antibodyPEBP2aB antibodyPolyomavirus enhancer binding protein 2 alpha B subunit antibodyPolyomavirus enhancer-binding protein 2 alpha B subunit antibodyRun1 antibodyRunt related transcription factor 1 antibodyRunt-related transcription factor 1 antibodyRUNX 1 antibodyRunx1 antibodyRUNX1_HUMAN antibodySL3 3 enhancer factor 1 alpha B subunit antibodySL3-3 enhancer factor 1 alpha B subunit antibodySL3/AKV core binding factor alpha B subunit antibodySL3/AKV core-binding factor alpha B subunit antibody
    see all

References for Anti-RUNX1 / AML1 antibody (ab55998)

ab55998 has not yet been referenced specifically in any publications.

Publishing research using ab55998 ? Please let us know so that we can cite the reference in this datasheet

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Votes: Highest First

Inquiry: Hello, We are interested in finding a RUNX1 antibody that can pick up all three isoforms: AML1A, AML1B, and AML1C [ transcript variants 1-3 see link We are planning to use it for WB and IP. On your website, I've found Ab 126469 – claims to ...

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Thanks for your enquiry.

I was just wondering which species of Runx1 you will be working with?

Thanks for the additional information!

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"