Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RUNX1 / AML1 antibody (ab61753)

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue with ab61753 at 1/50 -1/100 dilution, with and without the immunising peptide.

Western blot - RUNX1 / AML1 antibody (ab61753)

All lanes : Anti-RUNX1 / AML1 antibody (ab61753) at 1/500 dilution

Lane 1 : Jurkat cell extract
Lane 2 : Jurkat cell extract with the immunising peptide


Predicted band size : 49 kDa
Observed band size : 49 kDa
Additional bands at : 55 kDa,65 kDa,75 kDa. We are unsure as to the identity of these extra bands.

Immunohistochemistry (Frozen sections) - RUNX1 / AML1 antibody (ab61753)

ab61753 at a 1/200 dilution staining RUNX1/AML1 in mouse liver tissue sections by Immunohistochemistry (frozen sections) incubated for 9 hours at +4°C. Acetone fixed, permeabilized with 0.2% Triton X-100. Blocked using 2% BSA for 30 minutes at 20°C. Secondary used at 1/200 polyclonal Goat anti-rabbit IgG conjugated to Alexa Fluor 488 (green). Nuclei stained with DAPI (blue.)

This image is courtesy of an anonymous abreview.

Immunocytochemistry/ Immunofluorescence - Anti-RUNX1 / AML1 antibody (ab61753)

ICC/IF image of ab61753 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61753, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immunocytochemistry/ Immunofluorescence - Anti-RUNX1 / AML1 antibody (ab61753)

ab61753 staining RUNX1 / AML1 in rat glioblastoma cell line C6 by Immunocytochemistry/ Immunofluorescence.

Cells were fixed in paraformaldehyde, permeabilized using 0,1% Triton X 100 in PBS, blocked with 0.5% BSA for 30 minutes at room temperature and then incubated with ab61753 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a Cy3 conjugated goat anti-rabbit polyclonal used at a 1/400 dilution. Nuclei are counterstained with DAPI.

Image courtesy of an anonymous Abreview.