Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Neuroscience >> Neurology process >> Neurogenesis
Overview
Product name
Anti-RUNX1 / AML1 antibody
See all RUNX1 / AML1 products (14) ...
Description
Rabbit polyclonal to RUNX1 / AML1
Specificity
ab61753 detects endogenous levels of total AML1 protein.
Tested applications
WB, IHC-P, ELISA, IHC-Fr, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic non-phosphopeptide derived from human RUNX1/AML1 from around the phosphorylation site of Serine 276 (Q-P-SP-P-P).
Positive control
Human lung carcinoma tissue and Jurkat cell extract.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Organogenesis >> Hematopoietic system development
Stem Cells >> Hematopoietic Progenitors >> Hemangioblast
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Transcription factors
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Stem Cells >> Hematopoietic Progenitors >> Intracellular Molecules
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Neuroscience >> Neurology process >> Neurogenesis
Applications
Show applications key
Our Abpromise guarantee covers the use of ab61753 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/40,000ELISA: 1/40,000
IHC-Fr: Use at an assay dependent dilution. (See Abreview.)
ICC/IF: 1/200(See Abreview.)
Target
Function
CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits MYST4-dependent transcriptional activation.
Tissue specificity
Expressed in all tissues examined except brain and heart. Highest levels in thymus, bone marrow and peripheral blood.
Involvement in disease
Note=A chromosomal aberration involving RUNX1/AML1 is a cause of M2 type acute myeloid leukemia (AML-M2). Translocation t(8;21)(q22;q22) with RUNX1T1.
Note=A chromosomal aberration involving RUNX1/AML1 is a cause of therapy-related myelodysplastic syndrome (T-MDS). Translocation t(3;21)(q26;q22) with EAP or MECOM.
Note=A chromosomal aberration involving RUNX1/AML1 is a cause of chronic myelogenous leukemia (CML). Translocation t(3;21)(q26;q22) with EAP or MECOM.
Note=A chromosomal aberration involving RUNX1/AML1 is found in childhood acute lymphoblastic leukemia (ALL). Translocation t(12;21)(p13;q22) with TEL. The translocation fuses the 3'-end of TEL to the alternate 5'-exon of AML-1H.
Note=A chromosomal aberration involving RUNX1 is found in acute leukemia. Translocation t(11,21)(q13;q22) that forms a MACROD1-RUNX1 fusion protein.
Defects in RUNX1 are the cause of familial platelet disorder with associated myeloid malignancy (FPDMM) [MIM:601399]. FPDMM is an autosomal dominant disease characterized by qualitative and quantitative platelet defects, and propensity to develop acute myelogenous leukemia.
Note=A chromosomal aberration involving RUNX1/AML1 is found in therapy-related myeloid malignancies. Translocation t(16;21)(q24;q22) that forms a RUNX1-CBFA2T3 fusion protein.
Note=A chromosomal aberration involving RUNX1/AML1 is a cause of chronic myelomonocytic leukemia. Inversion inv(21)(q21;q22) with USP16.
Sequence similarities
Contains 1 Runt domain.
Domain
A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes.
Post-translational
modifications
Phosphorylated in its C-terminus upon IL-6 treatment. Phosphorylation enhances interaction with MYST3.
Methylated.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ01196
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Acute myeloid leukemia 1 antibody
- Acute myeloid leukemia 1 protein antibody
- alpha subunit core binding factor antibody
see all
Database links
- Entrez Gene: 861 Human
- Entrez Gene: 12394 Mouse
- Entrez Gene: 50662 Rat
- Omim: 151385 Human
- SwissProt: Q01196 Human
- SwissProt: Q03347 Mouse
- SwissProt: Q63046 Rat
- Unigene: 149261 Human
see all
Anti-RUNX1 / AML1 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RUNX1 / AML1 antibody (ab61753)
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue with ab61753 at 1/50 -1/100 dilution, with and without the immunising peptide.
Western blot - RUNX1 / AML1 antibody (ab61753)
All lanes : Anti-RUNX1 / AML1 antibody (ab61753) at 1/500 dilution
Lane 1 : Jurkat cell extract
Lane 2 : Jurkat cell extract with the immunising peptide
Predicted band size : 49 kDa
Observed band size : 49 kDa
Additional bands at : 55 kDa,65 kDa,75 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Frozen sections) - RUNX1 / AML1 antibody (ab61753)
ab61753 at a 1/200 dilution staining RUNX1/AML1 in mouse liver tissue sections by Immunohistochemistry (frozen sections) incubated for 9 hours at +4°C. Acetone fixed, permeabilized with 0.2% Triton X-100. Blocked using 2% BSA for 30 minutes at 20°C. Secondary used at 1/200 polyclonal Goat anti-rabbit IgG conjugated to Alexa Fluor 488 (green). Nuclei stained with DAPI (blue.)
This image is courtesy of an anonymous abreview.
Immunocytochemistry/ Immunofluorescence-RUNX1 / AML1 antibody(ab61753)
ICC/IF image of ab61753 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61753, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-RUNX1 / AML1 antibody (ab61753)
This product has been referenced in:
- Nagamachi Aet al. A 5' untranslated region containing the IRES element in the Runx1 gene is required for angiogenesis, hematopoiesis and leukemogenesis in a knock-in mouse model. Dev Biol 345:226-36 (2010). IHC-P; Mouse.Read more (PubMed: 20647008) »
See 1 publication for this product
Publishing research using ab61753? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"