Anti-RUNX2 antibody (ab11906)

Overview

• Product nameAnti-RUNX2 antibodySee all RUNX2 primary antibodies ...
• Description
  Rabbit polyclonal to RUNX2
• Tested applicationsEMSA more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  Synthetic peptide: TDVPRRISDDDTATSD, corresponding to amino acids 333-348 of Human AML3/Runx2.

  Run BLAST with Run BLAST with
• Positive controlSaos-2 nuclear extract.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.1% Sodium Azide and 0.01% Thiomerosal.
  Constituents: PBS containing 0.2% Gelatin.
• PurityWhole antiserum
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Developmental Biology
  • Organogenesis
  • Skeletal development
  • Bone

  • Stem Cells
  • Mesenchymal Stem Cells
  • Osteogenesis

  • Other

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Transcription Factors

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

Applications

Target

• FunctionTranscription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters (By similarity). Inhibits MYST4-dependent transcriptional activation.
• Tissue specificitySpecifically expressed in osteoblasts.
• Involvement in diseaseDefects in RUNX2 are the cause of cleidocranial dysplasia (CLCD) [MIM:119600]; also known as cleidocranial dysostosis (CCD). CLCD is an autosomal dominant skeletal disorder with high penetrance and variable expressivity. It is due to defective endochondral and intramembranous bone formation. Typical features include hypoplasia/aplasia of clavicles, patent fontanelles, wormian bones (additional cranial plates caused by abnormal ossification of the calvaria), supernumerary teeth, short stature, and other skeletal changes. In some cases defects in RUNX2 are exclusively associated with dental anomalies.
• Sequence similaritiesContains 1 Runt domain.
• DomainA proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes and contains the phosphorylation sites.
• Post-translational
  modificationsPhosphorylated; probably by MAP kinases (MAPK) (By similarity). Isoform 3 is phosphorylated on Ser-340.
• Cellular localizationNucleus.

Target information above from: UniProt accession Q13950 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 860 Human
  • Entrez Gene: 12393 Mouse
  • Omim: 600211 Human
  • SwissProt: Q13950 Human
  • SwissProt: Q08775 Mouse
  • Unigene: 535845 Human
  • Unigene: 391013 Mouse
  • Unigene: 391017 Mouse

• Alternative names
  Acute myeloid leukemia 3 protein antibodyalpha subunit 1 antibodyAML3 antibody

  CBF alpha 1 antibodyCBF-alpha-1 antibodyCBFA1 antibodyCCD antibodyCCD1 antibodycleidocranial dysplasia 1 antibodyCore binding factor antibodyCore binding factor runt domain alpha subunit 1 antibodyCore binding factor subunit alpha 1 antibodycore-binding factor antibodyCore-binding factor subunit alpha-1 antibodyMGC120022 antibodyMGC120023 antibodyOncogene AML 3 antibodyOncogene AML-3 antibodyOSF 2 antibodyOSF-2 antibodyOSF2 antibodyOsteoblast specific transcription factor 2 antibodyOsteoblast-specific transcription factor 2 antibodyOTTHUMP00000016533 antibodyPEA2 alpha A antibodyPEA2-alpha A antibodyPEA2aA antibodyPEBP2 alpha A antibodyPEBP2-alpha A antibodyPEBP2A1 antibodyPEBP2A2 antibodyPEBP2aA antibodyPEBP2aA antibodyPEBP2aA1 antibodyPolyomavirus enhancer binding protein 2 alpha A subunit antibodyPolyomavirus enhancer-binding protein 2 alpha A subunit antibodyrunt domain antibodyRunt related transcription factor 2 antibodyrunt-related transcription factor 2 antibodyRUNX2 antibodyRUNX2_HUMAN antibodySL3 3 enhancer factor 1 alpha A subunit antibodySL3-3 enhancer factor 1 alpha A subunit antibodySL3/AKV core binding factor alpha A subunit antibodySL3/AKV core-binding factor alpha A subunit antibody

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Anti-RUNX2 antibody images

• 

  Western blot - RUNX2 antibody (ab11906)
  Nushift polyclonal antibody against AML3 factor creates a supershift in EMSA (lane 4).