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Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human RUNX2.
(Peptide available as ab26346.)
Our Abpromise guarantee covers the use of ab23981 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. PubMed: 19092814|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 57 kDa).|
|EMSA||Use at an assay dependent concentration.|
ab23981 at a 1/1000 dilution staining RUNX2 in human U2-OS cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton X-100. Samples were blocked using 2% serum. The secondary used was an Alexa Fluor® 488 conjugated chicken anti-mouse at a 1/1000 dilution.